Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30541 | 5' | -52.1 | NC_006548.1 | + | 14974 | 0.66 | 0.783576 |
Target: 5'- aGCCGUGAUGACcugcAGGCG-CGcCUGUu -3' miRNA: 3'- aUGGCGCUGUUGuu--UCCGCaGU-GACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 28667 | 0.66 | 0.783576 |
Target: 5'- cGCCGCcugauGGCGugAAGGGUGUCuugGUg -3' miRNA: 3'- aUGGCG-----CUGUugUUUCCGCAGugaCG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 36286 | 0.66 | 0.783576 |
Target: 5'- cGCgGCGGCAcugagcgauGCAGAucuGGCGcaGCUGCa -3' miRNA: 3'- aUGgCGCUGU---------UGUUU---CCGCagUGACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 29482 | 0.66 | 0.773021 |
Target: 5'- --gCGCGGCAACGucGGCcagcgaUCGCUGa -3' miRNA: 3'- augGCGCUGUUGUuuCCGc-----AGUGACg -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 34236 | 0.66 | 0.773021 |
Target: 5'- gUugCGUGACGGCAAcaGCGUgACaggUGCa -3' miRNA: 3'- -AugGCGCUGUUGUUucCGCAgUG---ACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 23384 | 0.66 | 0.773021 |
Target: 5'- cACCGgGugGACcAGGGCcaUCGCUcGCu -3' miRNA: 3'- aUGGCgCugUUGuUUCCGc-AGUGA-CG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 20055 | 0.66 | 0.762304 |
Target: 5'- gGCCGaUGACGAgcGGGGCcUCGCUGa -3' miRNA: 3'- aUGGC-GCUGUUguUUCCGcAGUGACg -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 38284 | 0.66 | 0.762304 |
Target: 5'- gGCCGaUGGCGACAuAGuCGUC-CUGUg -3' miRNA: 3'- aUGGC-GCUGUUGUuUCcGCAGuGACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 29624 | 0.66 | 0.740436 |
Target: 5'- gGCCGCGAaugggcucGCAGGugcuGGUGcCGCUGCc -3' miRNA: 3'- aUGGCGCUgu------UGUUU----CCGCaGUGACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 1181 | 0.66 | 0.739328 |
Target: 5'- gGCCGCgcaGACGgccaggcucgcucGCGAauuGGGCGUCagcgaugcugaGCUGCg -3' miRNA: 3'- aUGGCG---CUGU-------------UGUU---UCCGCAG-----------UGACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 37517 | 0.67 | 0.730429 |
Target: 5'- aGCCGUGGCGGCAuguacucccaaauuGGCGU-ACUGg -3' miRNA: 3'- aUGGCGCUGUUGUuu------------CCGCAgUGACg -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 21058 | 0.67 | 0.729312 |
Target: 5'- cGCuCGCG-CuGCAAuGGCGUgagCACUGCc -3' miRNA: 3'- aUG-GCGCuGuUGUUuCCGCA---GUGACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 36635 | 0.67 | 0.729312 |
Target: 5'- gGCCGCGGCGuuGAucGCGUCGacGCg -3' miRNA: 3'- aUGGCGCUGUugUUucCGCAGUgaCG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 32940 | 0.67 | 0.729312 |
Target: 5'- gACCGCGuCGGCGAuucaacuGGCG-CGCccggUGCa -3' miRNA: 3'- aUGGCGCuGUUGUUu------CCGCaGUG----ACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 29438 | 0.67 | 0.705615 |
Target: 5'- aUACCGCGgccuccuGCAGCGAcaGGGUGcCAUcgGCg -3' miRNA: 3'- -AUGGCGC-------UGUUGUU--UCCGCaGUGa-CG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 7940 | 0.67 | 0.695344 |
Target: 5'- cGCCGCcuGCAuCAuccGGCG-CGCUGCg -3' miRNA: 3'- aUGGCGc-UGUuGUuu-CCGCaGUGACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 4491 | 0.67 | 0.683867 |
Target: 5'- gAUCGCGGCGAUGAAucuGGCGgugCAUgucgGCg -3' miRNA: 3'- aUGGCGCUGUUGUUU---CCGCa--GUGa---CG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 27237 | 0.68 | 0.660761 |
Target: 5'- cUGCCgagGCGGCAGCcAAGGCu--GCUGCu -3' miRNA: 3'- -AUGG---CGCUGUUGuUUCCGcagUGACG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 28600 | 0.68 | 0.659601 |
Target: 5'- cGCCGCGACcuggccggcgccgAGCAGGacuGCGUCAC-GCu -3' miRNA: 3'- aUGGCGCUG-------------UUGUUUc--CGCAGUGaCG- -5' |
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30541 | 5' | -52.1 | NC_006548.1 | + | 35511 | 0.68 | 0.649156 |
Target: 5'- gGCCGUGGCAaggaucGCGGccugcuggaccAGGCGUCAUaucGCa -3' miRNA: 3'- aUGGCGCUGU------UGUU-----------UCCGCAGUGa--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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