Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30553 | 5' | -57.7 | NC_006548.1 | + | 28087 | 1.09 | 0.000334 |
Target: 5'- gGCCUGCAGGCCGAACUCAACCGCCUGc -3' miRNA: 3'- -CGGACGUCCGGCUUGAGUUGGCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 28136 | 0.87 | 0.01573 |
Target: 5'- gGCCUGCAGGCCGc---CAGCCGCCUGc -3' miRNA: 3'- -CGGACGUCCGGCuugaGUUGGCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 28090 | 0.74 | 0.139648 |
Target: 5'- uCCUGCAGggccgcGCCGAaccgcggcagaaACUCGGCCGCCg- -3' miRNA: 3'- cGGACGUC------CGGCU------------UGAGUUGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 36416 | 0.74 | 0.143633 |
Target: 5'- aUCUGCAGGCCGGACg--GCCGCa-- -3' miRNA: 3'- cGGACGUCCGGCUUGaguUGGCGgac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 18735 | 0.73 | 0.156221 |
Target: 5'- aGCC-GCAGGCCGugcuGCUCGAC-GCCa- -3' miRNA: 3'- -CGGaCGUCCGGCu---UGAGUUGgCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 22200 | 0.73 | 0.156657 |
Target: 5'- cGCCUGgGGGCCGcacGCgucuuucaucuaccgCGAUCGCCUGg -3' miRNA: 3'- -CGGACgUCCGGCu--UGa--------------GUUGGCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 24017 | 0.73 | 0.164702 |
Target: 5'- cGCCUGcCAGGCCGGGCggugucCAGCCgaucaggGCCUu -3' miRNA: 3'- -CGGAC-GUCCGGCUUGa-----GUUGG-------CGGAc -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 965 | 0.72 | 0.181411 |
Target: 5'- aCCUGCgccAGGCUGAGCaguugcugacugaguUCAAgCGCCUGg -3' miRNA: 3'- cGGACG---UCCGGCUUG---------------AGUUgGCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 36368 | 0.72 | 0.189538 |
Target: 5'- gGCCUGCAGaugaacuacgcGCU--GCUCAGCCGCgUGg -3' miRNA: 3'- -CGGACGUC-----------CGGcuUGAGUUGGCGgAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 32899 | 0.71 | 0.217025 |
Target: 5'- cGCCaGCGGGUauCGcACUCGACCGUUUGu -3' miRNA: 3'- -CGGaCGUCCG--GCuUGAGUUGGCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 24446 | 0.7 | 0.282215 |
Target: 5'- cGCCUGCAauauGGCCGcuugcagcgcGGCaaGACCGCCa- -3' miRNA: 3'- -CGGACGU----CCGGC----------UUGagUUGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 17126 | 0.7 | 0.282215 |
Target: 5'- aUUUGCAGGCCGAugUUcGCCuugaCCUGg -3' miRNA: 3'- cGGACGUCCGGCUugAGuUGGc---GGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 13566 | 0.7 | 0.282215 |
Target: 5'- uGCCUGCGcGGgCGGcggcaagcugcuGCUCAACCagauccagaaaaGCCUGg -3' miRNA: 3'- -CGGACGU-CCgGCU------------UGAGUUGG------------CGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 14353 | 0.69 | 0.289525 |
Target: 5'- cGCCUGCAGGuCCGcgaUCAGCuCGUCc- -3' miRNA: 3'- -CGGACGUCC-GGCuugAGUUG-GCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 25126 | 0.69 | 0.296231 |
Target: 5'- gGCCgggGCGGGCUGGgucuugcGCUCAACCuugcGCCc- -3' miRNA: 3'- -CGGa--CGUCCGGCU-------UGAGUUGG----CGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 22222 | 0.69 | 0.304589 |
Target: 5'- uUCUGCcccGGCCGAGCgauguUCAGCCGCgCUu -3' miRNA: 3'- cGGACGu--CCGGCUUG-----AGUUGGCG-GAc -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 5724 | 0.68 | 0.336501 |
Target: 5'- aGCCUGgAccaCGGACUCGAUgGCCUGg -3' miRNA: 3'- -CGGACgUccgGCUUGAGUUGgCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 9245 | 0.68 | 0.336501 |
Target: 5'- cGCCUGUcgauugaucacGGGCUGGAuCUgGACgGCCUa -3' miRNA: 3'- -CGGACG-----------UCCGGCUU-GAgUUGgCGGAc -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 21361 | 0.68 | 0.336501 |
Target: 5'- gGCgaGCGGuuGCCGcGACUCuGCCGCCg- -3' miRNA: 3'- -CGgaCGUC--CGGC-UUGAGuUGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 18986 | 0.68 | 0.344851 |
Target: 5'- cGCCaUGUAGGCCcccgagccgGAACUggcgauuuccCGGCCGCCa- -3' miRNA: 3'- -CGG-ACGUCCGG---------CUUGA----------GUUGGCGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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