Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30553 | 5' | -57.7 | NC_006548.1 | + | 476 | 0.67 | 0.426496 |
Target: 5'- aGCgaGCuGGCCGAACU-GAUCGUCg- -3' miRNA: 3'- -CGgaCGuCCGGCUUGAgUUGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 965 | 0.72 | 0.181411 |
Target: 5'- aCCUGCgccAGGCUGAGCaguugcugacugaguUCAAgCGCCUGg -3' miRNA: 3'- cGGACG---UCCGGCUUG---------------AGUUgGCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 1035 | 0.68 | 0.361992 |
Target: 5'- gGCCUGCAGGaCGAugUCuAUCGgCa- -3' miRNA: 3'- -CGGACGUCCgGCUugAGuUGGCgGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 4882 | 0.68 | 0.353348 |
Target: 5'- uGCCUcCGGGCuCGAuUUCGGCCGCUc- -3' miRNA: 3'- -CGGAcGUCCG-GCUuGAGUUGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 5472 | 0.66 | 0.486886 |
Target: 5'- cGCCggGCucAGGUCGAccGCUCgAGCCaggucgGCCUGg -3' miRNA: 3'- -CGGa-CG--UCCGGCU--UGAG-UUGG------CGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 5724 | 0.68 | 0.336501 |
Target: 5'- aGCCUGgAccaCGGACUCGAUgGCCUGg -3' miRNA: 3'- -CGGACgUccgGCUUGAGUUGgCGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 7280 | 0.66 | 0.47653 |
Target: 5'- -gCUGCcGGUCGAGgUCAACCagGCgCUGg -3' miRNA: 3'- cgGACGuCCGGCUUgAGUUGG--CG-GAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 7416 | 0.66 | 0.466284 |
Target: 5'- aGCuCUGCAGGCagguCGAACUCGuugcguACCuCCUc -3' miRNA: 3'- -CG-GACGUCCG----GCUUGAGU------UGGcGGAc -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 7520 | 0.66 | 0.466284 |
Target: 5'- gGCCUGCucGGCC-AACUUGGCgaaGCCUu -3' miRNA: 3'- -CGGACGu-CCGGcUUGAGUUGg--CGGAc -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 7569 | 0.66 | 0.446143 |
Target: 5'- gGCCgaGCAGGCCGAggagcaagguGC-CGACCaucgcGCCUu -3' miRNA: 3'- -CGGa-CGUCCGGCU----------UGaGUUGG-----CGGAc -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 9245 | 0.68 | 0.336501 |
Target: 5'- cGCCUGUcgauugaucacGGGCUGGAuCUgGACgGCCUa -3' miRNA: 3'- -CGGACG-----------UCCGGCUU-GAgUUGgCGGAc -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 13566 | 0.7 | 0.282215 |
Target: 5'- uGCCUGCGcGGgCGGcggcaagcugcuGCUCAACCagauccagaaaaGCCUGg -3' miRNA: 3'- -CGGACGU-CCgGCU------------UGAGUUGG------------CGGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 14353 | 0.69 | 0.289525 |
Target: 5'- cGCCUGCAGGuCCGcgaUCAGCuCGUCc- -3' miRNA: 3'- -CGGACGUCC-GGCuugAGUUG-GCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 14464 | 0.66 | 0.436256 |
Target: 5'- cGCCucggUGCuGGCaCGAAUUgccgcCAGCCGCCg- -3' miRNA: 3'- -CGG----ACGuCCG-GCUUGA-----GUUGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 15242 | 0.67 | 0.42456 |
Target: 5'- --gUGCAGGgCGAACUCGuccaggaugcguCCGCCg- -3' miRNA: 3'- cggACGUCCgGCUUGAGUu-----------GGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 15401 | 0.67 | 0.388796 |
Target: 5'- cGUgUGCAGGCUGAuAUUCuucggauuGCCGCCc- -3' miRNA: 3'- -CGgACGUCCGGCU-UGAGu-------UGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 15671 | 0.68 | 0.352492 |
Target: 5'- cGCCUGCGaacagucGGCCGccCUCGggcuGCUGCCa- -3' miRNA: 3'- -CGGACGU-------CCGGCuuGAGU----UGGCGGac -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 16409 | 0.66 | 0.47653 |
Target: 5'- gGCCagcgGCAauuGGCCGuAgUCAGCCGCgaGu -3' miRNA: 3'- -CGGa---CGU---CCGGCuUgAGUUGGCGgaC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 17126 | 0.7 | 0.282215 |
Target: 5'- aUUUGCAGGCCGAugUUcGCCuugaCCUGg -3' miRNA: 3'- cGGACGUCCGGCUugAGuUGGc---GGAC- -5' |
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30553 | 5' | -57.7 | NC_006548.1 | + | 18034 | 0.66 | 0.486886 |
Target: 5'- -gCUGCAGGCggucaaGGGCgagCGACCGuUCUGg -3' miRNA: 3'- cgGACGUCCGg-----CUUGa--GUUGGC-GGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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