Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30686 | 3' | -52.7 | NC_006552.1 | + | 8277 | 0.67 | 0.788875 |
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Target: 5'- ---cCGCGCCCGcgcuGGACUGGCGcaacuaccccgAUGCg -3' miRNA: 3'- uguuGCGUGGGUc---CUUGACCGU-----------UACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 8056 | 0.66 | 0.853993 |
|
Target: 5'- gACAACcaGCA-CCGGGAcCUGGCg--GCg -3' miRNA: 3'- -UGUUG--CGUgGGUCCUuGACCGuuaCG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 4308 | 0.72 | 0.538373 |
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Target: 5'- uGCGGCGCACC--GGcGCUGGCAuaucggGCg -3' miRNA: 3'- -UGUUGCGUGGguCCuUGACCGUua----CG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 3604 | 0.69 | 0.715551 |
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Target: 5'- cGCAucuACGuCGCCuacgaCAGGGACUGGgAcgGCa -3' miRNA: 3'- -UGU---UGC-GUGG-----GUCCUUGACCgUuaCG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 2058 | 0.66 | 0.836445 |
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Target: 5'- cGCGGCGaaCACCCGcacaccgcGGAGCaGGCGAaccUGCc -3' miRNA: 3'- -UGUUGC--GUGGGU--------CCUUGaCCGUU---ACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 1505 | 0.76 | 0.327022 |
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Target: 5'- cCGACGCaagccGCCCAGGAcCUGGCAcuccGCa -3' miRNA: 3'- uGUUGCG-----UGGGUCCUuGACCGUua--CG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 1305 | 1.12 | 0.001266 |
|
Target: 5'- gACAACGCACCCAGGAACUGGCAAUGCu -3' miRNA: 3'- -UGUUGCGUGGGUCCUUGACCGUUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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