Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30697 | 3' | -55.6 | NC_006552.1 | + | 49118 | 0.69 | 0.572697 |
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Target: 5'- ---aGCCCCAuCugCUcggagcgcuuugggGCCGCCGGGACc -3' miRNA: 3'- cgagUGGGGU-GugGA--------------UGGCGGCUUUG- -5' |
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| 30697 | 3' | -55.6 | NC_006552.1 | + | 18666 | 0.69 | 0.575915 |
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Target: 5'- cUUCAgCCCGCGCCUgGCCGagGAGAUa -3' miRNA: 3'- cGAGUgGGGUGUGGA-UGGCggCUUUG- -5' |
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| 30697 | 3' | -55.6 | NC_006552.1 | + | 27548 | 0.69 | 0.586673 |
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Target: 5'- cGCUCucuGCCuCCGCuuCUGCUGCCGGc-- -3' miRNA: 3'- -CGAG---UGG-GGUGugGAUGGCGGCUuug -5' |
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| 30697 | 3' | -55.6 | NC_006552.1 | + | 4012 | 0.68 | 0.619135 |
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Target: 5'- cGCUCGgcaagCCUGCGCCgauaucgGCUGCUGggGCg -3' miRNA: 3'- -CGAGUg----GGGUGUGGa------UGGCGGCuuUG- -5' |
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| 30697 | 3' | -55.6 | NC_006552.1 | + | 13563 | 0.68 | 0.627818 |
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Target: 5'- cGCgagCGCCaggaacggaucgCACGCCUGCUGUCGAAGg -3' miRNA: 3'- -CGa--GUGGg-----------GUGUGGAUGGCGGCUUUg -5' |
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| 30697 | 3' | -55.6 | NC_006552.1 | + | 43204 | 0.68 | 0.629989 |
|
Target: 5'- uGCagCGCCCCAguCGCCUgAUCGCUGAcgguGGCg -3' miRNA: 3'- -CGa-GUGGGGU--GUGGA-UGGCGGCU----UUG- -5' |
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| 30697 | 3' | -55.6 | NC_006552.1 | + | 13932 | 0.68 | 0.629989 |
|
Target: 5'- aGCUUucCCCCGCuCCUACCaGCCccgaGGAAUg -3' miRNA: 3'- -CGAGu-GGGGUGuGGAUGG-CGG----CUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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