Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 30878 | 3' | -59.2 | NC_006552.1 | + | 63191 | 0.66 | 0.551099 |
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Target: 5'- -aGCGCUGCaaGGCGUUGuccuGGCCGCaaCGGu -3' miRNA: 3'- ggCGCGAUG--UCGUAGC----UCGGCG--GCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 33748 | 0.66 | 0.551099 |
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Target: 5'- gCCaGCGCggcgGCGGCAuugcugUUGAGCagaaCGCCGGc -3' miRNA: 3'- -GG-CGCGa---UGUCGU------AGCUCG----GCGGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 54115 | 0.66 | 0.540825 |
|
Target: 5'- aCCGCagGCUcugGC-GCAUCGucugagcgccgGGCCGcCCGGAg -3' miRNA: 3'- -GGCG--CGA---UGuCGUAGC-----------UCGGC-GGCCU- -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 26870 | 0.66 | 0.540825 |
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Target: 5'- gCCGCGUUGCAGCGcggcaucuUCG-GCgacguuguugaUGCCGGc -3' miRNA: 3'- -GGCGCGAUGUCGU--------AGCuCG-----------GCGGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 52442 | 0.66 | 0.540825 |
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Target: 5'- aCUGgGUUGaGGgAUgGGGCUGCCGGAu -3' miRNA: 3'- -GGCgCGAUgUCgUAgCUCGGCGGCCU- -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 37776 | 0.66 | 0.591664 |
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Target: 5'- cCCGCGCUcugcaucACAGCGUCGGcGaUCGgCGcGAu -3' miRNA: 3'- -GGCGCGA-------UGUCGUAGCU-C-GGCgGC-CU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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