Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31004 | 5' | -60.5 | NC_006560.1 | + | 129049 | 0.66 | 0.727574 |
Target: 5'- -uCCG-GGCCCAGCggcccCUCCgCGAg -3' miRNA: 3'- cuGGCaCCGGGUCGagau-GAGGgGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 113825 | 0.66 | 0.727574 |
Target: 5'- -cCCGUGGCgcuguaccaCCAGCUgcggacgggcCUGCaCCCCGGg -3' miRNA: 3'- cuGGCACCG---------GGUCGA----------GAUGaGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 105659 | 0.66 | 0.727574 |
Target: 5'- cGACCG-GGaCCCcggGGC-CUACUCCguCCGGg -3' miRNA: 3'- -CUGGCaCC-GGG---UCGaGAUGAGG--GGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 106582 | 0.66 | 0.727574 |
Target: 5'- gGGCCGgGGCCUGGC-Cg---CCCCGGu -3' miRNA: 3'- -CUGGCaCCGGGUCGaGaugaGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 68030 | 0.66 | 0.717938 |
Target: 5'- -cCCGacGGCCCgcugcaGGCUCUGCUgcgggCCCUGAc -3' miRNA: 3'- cuGGCa-CCGGG------UCGAGAUGA-----GGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 70722 | 0.66 | 0.708232 |
Target: 5'- cGGCgCGUGGCCgAGUUCguccgggaGCUCCaCGAc -3' miRNA: 3'- -CUG-GCACCGGgUCGAGa-------UGAGGgGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 39803 | 0.66 | 0.708232 |
Target: 5'- aGGCC--GGCCCgGGCUCcGCgUCCUCGAa -3' miRNA: 3'- -CUGGcaCCGGG-UCGAGaUG-AGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 71490 | 0.66 | 0.702379 |
Target: 5'- gGAUCGUGGUcaccggggcgaggaaCCAGUUC-GC-CCCCGAc -3' miRNA: 3'- -CUGGCACCG---------------GGUCGAGaUGaGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 61204 | 0.66 | 0.698465 |
Target: 5'- cGACCGU-GCCC-GCUC--CUCCCCc- -3' miRNA: 3'- -CUGGCAcCGGGuCGAGauGAGGGGcu -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 139185 | 0.66 | 0.688646 |
Target: 5'- uGCCGccGGCCCAGCUCg--UCCaCgGAg -3' miRNA: 3'- cUGGCa-CCGGGUCGAGaugAGG-GgCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 31190 | 0.66 | 0.678782 |
Target: 5'- cGCCGgcccGCCCGGCgCaGCUCCCCc- -3' miRNA: 3'- cUGGCac--CGGGUCGaGaUGAGGGGcu -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 142691 | 0.66 | 0.678782 |
Target: 5'- gGACCGgaggggGcGCCCGGCcggCcGCggCCCCGAg -3' miRNA: 3'- -CUGGCa-----C-CGGGUCGa--GaUGa-GGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 144879 | 0.66 | 0.676804 |
Target: 5'- --gCGUGGCCCugAGCgcccauguuCUCCCCGGc -3' miRNA: 3'- cugGCACCGGG--UCGagau-----GAGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 104214 | 0.67 | 0.668881 |
Target: 5'- cGGCCGUgcugucguucgcGGCCCuGCU--GC-CCCCGAc -3' miRNA: 3'- -CUGGCA------------CCGGGuCGAgaUGaGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 45898 | 0.67 | 0.668881 |
Target: 5'- cACCGggGGCgaCCGGCggguCUCCCCGGg -3' miRNA: 3'- cUGGCa-CCG--GGUCGagauGAGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 47480 | 0.67 | 0.658952 |
Target: 5'- uGGCaGcGGCCCAGCUCUGggacCUCuCCCa- -3' miRNA: 3'- -CUGgCaCCGGGUCGAGAU----GAG-GGGcu -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 115878 | 0.67 | 0.649003 |
Target: 5'- -cCCGUGGCCCGGgUgUGCacguucgacggCCCCGc -3' miRNA: 3'- cuGGCACCGGGUCgAgAUGa----------GGGGCu -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 69120 | 0.67 | 0.649003 |
Target: 5'- cGCCG-GcGCCCAGCggcgcCUGCUCgCCCu- -3' miRNA: 3'- cUGGCaC-CGGGUCGa----GAUGAG-GGGcu -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 52599 | 0.67 | 0.649003 |
Target: 5'- aGGCgGUGGCgaCgGGCgggCUGCggaUCCCCGAg -3' miRNA: 3'- -CUGgCACCG--GgUCGa--GAUG---AGGGGCU- -5' |
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31004 | 5' | -60.5 | NC_006560.1 | + | 73175 | 0.67 | 0.633061 |
Target: 5'- cGCCGgcgcgggggcggagGGCCCGGcCUCggccCUCCUCGAc -3' miRNA: 3'- cUGGCa-------------CCGGGUC-GAGau--GAGGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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