Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31298 | 3' | -44.9 | NC_006623.1 | + | 139699 | 0.71 | 0.998687 |
Target: 5'- gGGGGugGGAGGGAGGg--GGAGg--- -3' miRNA: 3'- -CCUCugUCUUUCUCCuuaCCUUaacc -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 137767 | 0.71 | 0.998404 |
Target: 5'- cGGAGuuAGuuGGGGGGccgaacggGGAGUUGGa -3' miRNA: 3'- -CCUCugUCuuUCUCCUua------CCUUAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 121372 | 0.71 | 0.99796 |
Target: 5'- cGGAGuCGGAggaaGAGcagccugggugccgGGGAGUGGAcUUGGg -3' miRNA: 3'- -CCUCuGUCU----UUC--------------UCCUUACCUuAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 140240 | 0.71 | 0.99796 |
Target: 5'- cGGAGuCGGAggaaGAGcagccugggugccgGGGAGUGGAcUUGGg -3' miRNA: 3'- -CCUCuGUCU----UUC--------------UCCUUACCUuAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 47355 | 0.72 | 0.997179 |
Target: 5'- uGGGGGCGGAAGGAuaGGAAcgcagauUGGAA--GGg -3' miRNA: 3'- -CCUCUGUCUUUCU--CCUU-------ACCUUaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 109050 | 0.73 | 0.991584 |
Target: 5'- cGGAGACAGaAGAGGGGGAaGGcaacuaUGGc -3' miRNA: 3'- -CCUCUGUC-UUUCUCCUUaCCuua---ACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 112200 | 0.75 | 0.981573 |
Target: 5'- aGGGGACuGGGAGGGGAcgGGuc--GGa -3' miRNA: 3'- -CCUCUGuCUUUCUCCUuaCCuuaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 124619 | 0.76 | 0.964521 |
Target: 5'- cGGAGGUAaaaauuuuGAGAGAGGAGUGGAAauauuuUUGGu -3' miRNA: 3'- -CCUCUGU--------CUUUCUCCUUACCUU------AACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 531 | 0.77 | 0.956945 |
Target: 5'- aGGAGACuGAuuGGGGAAUG--AUUGGg -3' miRNA: 3'- -CCUCUGuCUuuCUCCUUACcuUAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 880 | 0.83 | 0.744158 |
Target: 5'- cGGAGAgGGAGAuAGGAAUGGAAU-GGu -3' miRNA: 3'- -CCUCUgUCUUUcUCCUUACCUUAaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 740 | 1.14 | 0.019448 |
Target: 5'- uGGAGACAGAAAGAGGAAUGGAAUUGGc -3' miRNA: 3'- -CCUCUGUCUUUCUCCUUACCUUAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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