Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31298 | 3' | -44.9 | NC_006623.1 | + | 123067 | 0.7 | 0.999643 |
Target: 5'- cGGAGugGguGAGGGGGGAgaGUGGAc--GGa -3' miRNA: 3'- -CCUCugU--CUUUCUCCU--UACCUuaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 123844 | 0.71 | 0.998404 |
Target: 5'- cGGAGuuAGuuGGGGGGccgaacggGGAGUUGGa -3' miRNA: 3'- -CCUCugUCuuUCUCCUua------CCUUAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 124619 | 0.76 | 0.964521 |
Target: 5'- cGGAGGUAaaaauuuuGAGAGAGGAGUGGAAauauuuUUGGu -3' miRNA: 3'- -CCUCUGU--------CUUUCUCCUUACCUU------AACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 137767 | 0.71 | 0.998404 |
Target: 5'- cGGAGuuAGuuGGGGGGccgaacggGGAGUUGGa -3' miRNA: 3'- -CCUCugUCuuUCUCCUua------CCUUAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 138545 | 0.7 | 0.999643 |
Target: 5'- cGGAGugGguGAGGGGGGAgaGUGGAc--GGa -3' miRNA: 3'- -CCUCugU--CUUUCUCCU--UACCUuaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 139245 | 0.68 | 0.999944 |
Target: 5'- gGGAGAauUAG-AGGAGGcAAUGGAAg-GGa -3' miRNA: 3'- -CCUCU--GUCuUUCUCC-UUACCUUaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 139699 | 0.71 | 0.998687 |
Target: 5'- gGGGGugGGAGGGAGGg--GGAGg--- -3' miRNA: 3'- -CCUCugUCUUUCUCCuuaCCUUaacc -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 140240 | 0.71 | 0.99796 |
Target: 5'- cGGAGuCGGAggaaGAGcagccugggugccgGGGAGUGGAcUUGGg -3' miRNA: 3'- -CCUCuGUCU----UUC--------------UCCUUACCUuAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 143591 | 0.68 | 0.999944 |
Target: 5'- cGAGAaugAGGAAGAGGA--GGAAgaGGa -3' miRNA: 3'- cCUCUg--UCUUUCUCCUuaCCUUaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 146888 | 0.69 | 0.9999 |
Target: 5'- aGGAcGCugcgAGAGGGAGGGcgGGAAUUa- -3' miRNA: 3'- -CCUcUG----UCUUUCUCCUuaCCUUAAcc -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 147088 | 0.66 | 0.999996 |
Target: 5'- uGGGGACcGAuugcGGGGAGUGuGAuaaUGGg -3' miRNA: 3'- -CCUCUGuCUuu--CUCCUUAC-CUua-ACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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