Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31298 | 3' | -44.9 | NC_006623.1 | + | 146888 | 0.69 | 0.9999 |
Target: 5'- aGGAcGCugcgAGAGGGAGGGcgGGAAUUa- -3' miRNA: 3'- -CCUcUG----UCUUUCUCCUuaCCUUAAcc -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 143591 | 0.68 | 0.999944 |
Target: 5'- cGAGAaugAGGAAGAGGA--GGAAgaGGa -3' miRNA: 3'- cCUCUg--UCUUUCUCCUuaCCUUaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 139245 | 0.68 | 0.999944 |
Target: 5'- gGGAGAauUAG-AGGAGGcAAUGGAAg-GGa -3' miRNA: 3'- -CCUCU--GUCuUUCUCC-UUACCUUaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 122367 | 0.68 | 0.999944 |
Target: 5'- gGGAGAauUAG-AGGAGGcAAUGGAAg-GGa -3' miRNA: 3'- -CCUCU--GUCuUUCUCC-UUACCUUaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 118021 | 0.68 | 0.999944 |
Target: 5'- cGAGAaugAGGAAGAGGA--GGAAgaGGa -3' miRNA: 3'- cCUCUg--UCUUUCUCCUuaCCUUaaCC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 89914 | 0.68 | 0.999944 |
Target: 5'- aGAGuCAGAGAGcauuaaGAcUGGAGUUGGa -3' miRNA: 3'- cCUCuGUCUUUCuc----CUuACCUUAACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 550 | 0.68 | 0.999969 |
Target: 5'- aGGGGCAGcaguAGAGGAcaGUGGAGa--- -3' miRNA: 3'- cCUCUGUCuu--UCUCCU--UACCUUaacc -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 52106 | 0.67 | 0.999984 |
Target: 5'- aGGAGAUAcuGGAGGGGAAUGcGuGUUGu -3' miRNA: 3'- -CCUCUGUc-UUUCUCCUUAC-CuUAACc -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 524 | 0.67 | 0.999994 |
Target: 5'- aGAGACGGGGAagaagcggagcGAGGuagGGAGUgUGGc -3' miRNA: 3'- cCUCUGUCUUU-----------CUCCuuaCCUUA-ACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 147088 | 0.66 | 0.999996 |
Target: 5'- uGGGGACcGAuugcGGGGAGUGuGAuaaUGGg -3' miRNA: 3'- -CCUCUGuCUuu--CUCCUUAC-CUua-ACC- -5' |
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31298 | 3' | -44.9 | NC_006623.1 | + | 114524 | 0.66 | 0.999996 |
Target: 5'- uGGGGACcGAuugcGGGGAGUGuGAuaaUGGg -3' miRNA: 3'- -CCUCUGuCUuu--CUCCUUAC-CUua-ACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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