Results 1 - 20 of 60 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31659 | 3' | -59.6 | NC_006883.1 | + | 208989 | 0.66 | 0.870834 |
Target: 5'- -cGGuAGGGauuaCAGCAGGuucACUGGGACUAa -3' miRNA: 3'- gaCC-UCCUg---GUCGUCC---UGGUCCUGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 28513 | 0.66 | 0.863748 |
Target: 5'- -aGuuGGACCAGUcGGACCAGuuCCAc -3' miRNA: 3'- gaCcuCCUGGUCGuCCUGGUCcuGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 22607 | 0.66 | 0.849016 |
Target: 5'- uCUGGuacaacuGGAUCuGguGGAUCAGGugUAa -3' miRNA: 3'- -GACCu------CCUGGuCguCCUGGUCCugGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 223080 | 0.66 | 0.84138 |
Target: 5'- ------cACCAGCAGGACCAGGAg-- -3' miRNA: 3'- gaccuccUGGUCGUCCUGGUCCUggu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 27219 | 0.67 | 0.8256 |
Target: 5'- cCUGGuccuacAGGuCCAGCAGGuccuaCAGGuCCAu -3' miRNA: 3'- -GACC------UCCuGGUCGUCCug---GUCCuGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 210951 | 0.67 | 0.815823 |
Target: 5'- uCUGGAuGACCAGaaucuacaaguGGACCAGuuGGCCAa -3' miRNA: 3'- -GACCUcCUGGUCgu---------CCUGGUC--CUGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 23441 | 0.68 | 0.765737 |
Target: 5'- uCUGGu---CCAGCAGGuccuCCAGGuCCAg -3' miRNA: 3'- -GACCuccuGGUCGUCCu---GGUCCuGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 24961 | 0.69 | 0.710151 |
Target: 5'- aUGGAccacuuGGACCuGUAGGACCuggAGGAgCAa -3' miRNA: 3'- gACCU------CCUGGuCGUCCUGG---UCCUgGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 26978 | 0.69 | 0.69106 |
Target: 5'- cCUGGAGaACCAGCAGGACCuuGuGAaacugauacCCAc -3' miRNA: 3'- -GACCUCcUGGUCGUCCUGGu-C-CU---------GGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 27304 | 0.69 | 0.681443 |
Target: 5'- gUGGcacAGGuCCAGCAGGuccuCCAGGuCCu -3' miRNA: 3'- gACC---UCCuGGUCGUCCu---GGUCCuGGu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 27372 | 0.7 | 0.642686 |
Target: 5'- aCUGGuacuccAGGuCCAGCAGGuccuCCAGGuCCu -3' miRNA: 3'- -GACC------UCCuGGUCGUCCu---GGUCCuGGu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 28410 | 0.7 | 0.613514 |
Target: 5'- cCUGGAGGACCuGgAGGACCAcuuCCc -3' miRNA: 3'- -GACCUCCUGGuCgUCCUGGUccuGGu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 28178 | 0.71 | 0.555672 |
Target: 5'- ---cAGcACCAGCAGGACCAguuGGACCAg -3' miRNA: 3'- gaccUCcUGGUCGUCCUGGU---CCUGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 222992 | 0.72 | 0.527317 |
Target: 5'- -aGGcGaACCAGCAGGACCAGGugaACCu -3' miRNA: 3'- gaCCuCcUGGUCGUCCUGGUCC---UGGu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 23929 | 0.73 | 0.472391 |
Target: 5'- uUGGAccaguuGGACCuGUAGGACCugcuGGACCu -3' miRNA: 3'- gACCU------CCUGGuCGUCCUGGu---CCUGGu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 27745 | 0.73 | 0.454725 |
Target: 5'- cCUGuGGGACCAGUugguccuguGGGACCugucGGACCAg -3' miRNA: 3'- -GACcUCCUGGUCG---------UCCUGGu---CCUGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 23382 | 0.74 | 0.423857 |
Target: 5'- cCUGuGGGACCAGUuggaccuggauuugaAGGACCuacAGGACCAg -3' miRNA: 3'- -GACcUCCUGGUCG---------------UCCUGG---UCCUGGU- -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 28146 | 0.75 | 0.387887 |
Target: 5'- cCUGaAGGACCAGUuGGACCAguuGGACCu -3' miRNA: 3'- -GACcUCCUGGUCGuCCUGGU---CCUGGu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 22095 | 0.75 | 0.379996 |
Target: 5'- cCUGGAGGACCAcUuGGACCAGGAg-- -3' miRNA: 3'- -GACCUCCUGGUcGuCCUGGUCCUggu -5' |
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31659 | 3' | -59.6 | NC_006883.1 | + | 26710 | 0.75 | 0.36454 |
Target: 5'- -aGGAGGACCuGCuGGACCuGGAguaCCAg -3' miRNA: 3'- gaCCUCCUGGuCGuCCUGGuCCU---GGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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