Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31659 | 5' | -56.6 | NC_006883.1 | + | 212118 | 0.66 | 0.964728 |
Target: 5'- uUCUGGuGGAUauggaUACUuaaguagAGGACCUGGa -3' miRNA: 3'- -GGACCuCCUGgug--GUGA-------UCCUGGACC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 213907 | 0.67 | 0.942784 |
Target: 5'- aCUGGuaAGGACCACCAUugUAGaGCCa-- -3' miRNA: 3'- gGACC--UCCUGGUGGUG--AUCcUGGacc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 21800 | 0.67 | 0.938343 |
Target: 5'- --aGGAccaGGACCGCCACcuGGACCa-- -3' miRNA: 3'- ggaCCU---CCUGGUGGUGauCCUGGacc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 112315 | 0.68 | 0.906576 |
Target: 5'- aCUGGuGGAUCuggugguGCUACUGguGGAUCUGGu -3' miRNA: 3'- gGACCuCCUGG-------UGGUGAU--CCUGGACC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 211620 | 0.69 | 0.888685 |
Target: 5'- uCCUGGugcuacucuAGGACCACCACUccaaGAuCCUGc -3' miRNA: 3'- -GGACC---------UCCUGGUGGUGAuc--CU-GGACc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 21943 | 0.69 | 0.882112 |
Target: 5'- aCCaGGAGGACCAgUu---GGACCUGu -3' miRNA: 3'- -GGaCCUCCUGGUgGugauCCUGGACc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 26656 | 0.69 | 0.882112 |
Target: 5'- gUCUGGAccaguuGGACCaguuccACCugUGGGACCa-- -3' miRNA: 3'- -GGACCU------CCUGG------UGGugAUCCUGGacc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 28147 | 0.69 | 0.878072 |
Target: 5'- aCCUGaAGGACCaguuggaccaguuggACCugUAGGuCCUGu -3' miRNA: 3'- -GGACcUCCUGG---------------UGGugAUCCuGGACc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 26988 | 0.69 | 0.868368 |
Target: 5'- aCCaGGAGGACCuggagaACCAgcAGGACCUu- -3' miRNA: 3'- -GGaCCUCCUGG------UGGUgaUCCUGGAcc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 23916 | 0.69 | 0.868368 |
Target: 5'- aCCUGuAGGACCugCu---GGACCUGu -3' miRNA: 3'- -GGACcUCCUGGugGugauCCUGGACc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 15375 | 0.69 | 0.861205 |
Target: 5'- aCUUGGAaaauguuauaguGGACCuCCAUUAGGAUgugCUGGu -3' miRNA: 3'- -GGACCU------------CCUGGuGGUGAUCCUG---GACC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 23203 | 0.71 | 0.780273 |
Target: 5'- aCCaGGAGGACCacuuggACCAUcAGGACCa-- -3' miRNA: 3'- -GGaCCUCCUGG------UGGUGaUCCUGGacc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 22065 | 0.72 | 0.753318 |
Target: 5'- ---nGAGGACCACCAgcAGGACCaguUGGa -3' miRNA: 3'- ggacCUCCUGGUGGUgaUCCUGG---ACC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 21999 | 0.72 | 0.753318 |
Target: 5'- gUUGGAccaGGACCuCCACUuGGACCaGGa -3' miRNA: 3'- gGACCU---CCUGGuGGUGAuCCUGGaCC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 21845 | 0.73 | 0.677762 |
Target: 5'- --aGGAccaGGACCACCACUcGGACCa-- -3' miRNA: 3'- ggaCCU---CCUGGUGGUGAuCCUGGacc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 26794 | 0.73 | 0.658379 |
Target: 5'- aCCUGGuGGACCagcaggACCAgUuGGACCUGc -3' miRNA: 3'- -GGACCuCCUGG------UGGUgAuCCUGGACc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 23287 | 0.74 | 0.629179 |
Target: 5'- aCCUGGAGGACCuug---AGGACCaGGa -3' miRNA: 3'- -GGACCUCCUGGuggugaUCCUGGaCC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 23158 | 0.75 | 0.590303 |
Target: 5'- aCCUGGAGcACCagcaggACCAgUuGGACCUGGa -3' miRNA: 3'- -GGACCUCcUGG------UGGUgAuCCUGGACC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 23365 | 0.75 | 0.561413 |
Target: 5'- aCCUGGAuuugaaGGACCuacaggACCAgUuGGACCUGGa -3' miRNA: 3'- -GGACCU------CCUGG------UGGUgAuCCUGGACC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 23419 | 0.76 | 0.517938 |
Target: 5'- aCCUGuAGGACCaucugguccaguuggACCugUGGGACCUGu -3' miRNA: 3'- -GGACcUCCUGG---------------UGGugAUCCUGGACc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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