Results 1 - 20 of 60 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31660 | 5' | -50.5 | NC_006883.1 | + | 21748 | 0.66 | 0.999148 |
Target: 5'- cUGGuccUGcuGGuCCUACGGGuCCAGa -3' miRNA: 3'- -ACCuaaACuuCCuGGAUGUCCuGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 22138 | 0.66 | 0.998964 |
Target: 5'- nGG---UGAAccacuuGGACCaGCAGGACCAu -3' miRNA: 3'- aCCuaaACUU------CCUGGaUGUCCUGGUc -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 203460 | 0.66 | 0.998747 |
Target: 5'- gGGAUaUUGAaucuugggAGGAUCa--AGGACCAGa -3' miRNA: 3'- aCCUA-AACU--------UCCUGGaugUCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 211372 | 0.67 | 0.998194 |
Target: 5'- aGGAgguaauaAAGGACCU-CAuccuGGACCAGg -3' miRNA: 3'- aCCUaaac---UUCCUGGAuGU----CCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 23156 | 0.67 | 0.998194 |
Target: 5'- -----cUGGAGcACCaGCAGGACCAGu -3' miRNA: 3'- accuaaACUUCcUGGaUGUCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 19055 | 0.67 | 0.997849 |
Target: 5'- aGGAcUUUGAuaucuuccucuAGGACCUucguuggaAGGACCAa -3' miRNA: 3'- aCCU-AAACU-----------UCCUGGAug------UCCUGGUc -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 28160 | 0.67 | 0.996991 |
Target: 5'- uUGGAccagUUccaccUGAAGGACCaGuuGGACCAGn -3' miRNA: 3'- -ACCU----AA-----ACUUCCUGGaUguCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 223007 | 0.67 | 0.996991 |
Target: 5'- aGGAccagUUGcaccAGGcgaACCaGCAGGACCAGg -3' miRNA: 3'- aCCUa---AACu---UCC---UGGaUGUCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 22237 | 0.67 | 0.996466 |
Target: 5'- aGGAccaGGAGGACC-ACuuGGACCAu -3' miRNA: 3'- aCCUaaaCUUCCUGGaUGu-CCUGGUc -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 23145 | 0.68 | 0.995867 |
Target: 5'- cUGGAccaGAuGGuCCUACAGGuccACCAGg -3' miRNA: 3'- -ACCUaaaCUuCCuGGAUGUCC---UGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 248294 | 0.68 | 0.995188 |
Target: 5'- aGGAUgUGcuGGACUgagaucauUAGGACCAGa -3' miRNA: 3'- aCCUAaACuuCCUGGau------GUCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 26982 | 0.68 | 0.995188 |
Target: 5'- aGGAccUGGAGaACCaGCAGGACCu- -3' miRNA: 3'- aCCUaaACUUCcUGGaUGUCCUGGuc -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 27732 | 0.69 | 0.990313 |
Target: 5'- uUGGuccUGuGGGACCUGucGGACCAGu -3' miRNA: 3'- -ACCuaaACuUCCUGGAUguCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 24952 | 0.69 | 0.987519 |
Target: 5'- uUGGAccUGuAGGACCUGgAGGAgCAa -3' miRNA: 3'- -ACCUaaACuUCCUGGAUgUCCUgGUc -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 21940 | 0.7 | 0.98221 |
Target: 5'- aGGAg--GAccaguuGGACCUGuuGGACCAGc -3' miRNA: 3'- aCCUaaaCUu-----CCUGGAUguCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 27847 | 0.7 | 0.975358 |
Target: 5'- cUGGuccuacAGGACCUACAGGuCCAa -3' miRNA: 3'- -ACCuaaacuUCCUGGAUGUCCuGGUc -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 28124 | 0.71 | 0.972695 |
Target: 5'- uUGGAccUGuAGGuCCUGUAGGACCAGn -3' miRNA: 3'- -ACCUaaACuUCCuGGAUGUCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 27552 | 0.71 | 0.96983 |
Target: 5'- cUGGAccaccuGGACCUGgAGGACCAu -3' miRNA: 3'- -ACCUaaacuuCCUGGAUgUCCUGGUc -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 27758 | 0.71 | 0.963467 |
Target: 5'- aGGAccaGuuGGACCUGUGGGACCAGu -3' miRNA: 3'- aCCUaaaCuuCCUGGAUGUCCUGGUC- -5' |
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31660 | 5' | -50.5 | NC_006883.1 | + | 23206 | 0.71 | 0.962783 |
Target: 5'- aGGAccaGGAGGACCacuuggaccauCAGGACCAGc -3' miRNA: 3'- aCCUaaaCUUCCUGGau---------GUCCUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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