Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31790 | 5' | -56.3 | NC_006938.1 | + | 48352 | 0.66 | 0.666526 |
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Target: 5'- gGGCGGCGGUGaCGAGcGC-GUCCugUCGGa -3' miRNA: 3'- -CCGUCGUUACgGUUC-CGgCAGG--AGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 47010 | 0.66 | 0.666526 |
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Target: 5'- gGGUGGCAAcgacgcUGCCcugggcGGCCGaaUCUUCGAg -3' miRNA: 3'- -CCGUCGUU------ACGGuu----CCGGC--AGGAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 18594 | 0.66 | 0.677334 |
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Target: 5'- uGCGGC-AUGCCc-GGCgCGUCCgaggCGGc -3' miRNA: 3'- cCGUCGuUACGGuuCCG-GCAGGa---GCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 39543 | 0.66 | 0.6546 |
|
Target: 5'- cGGUGGCAcgGCCAccGcucgcacggucucGGCCGUCgacuuCUCGGa -3' miRNA: 3'- -CCGUCGUuaCGGU--U-------------CCGGCAG-----GAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 10671 | 0.67 | 0.633951 |
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Target: 5'- aGGaCGGCAAggugGUCGAGGgCGcaaUCCUCGc -3' miRNA: 3'- -CC-GUCGUUa---CGGUUCCgGC---AGGAGCu -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 1337 | 0.67 | 0.623077 |
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Target: 5'- uGGCAGCAagGCC---GCCGUCgccagagagCUCGGg -3' miRNA: 3'- -CCGUCGUuaCGGuucCGGCAG---------GAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 7576 | 0.67 | 0.601361 |
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Target: 5'- uGGCcaAGCAccugGCCGAGGgCGU-CUCGGg -3' miRNA: 3'- -CCG--UCGUua--CGGUUCCgGCAgGAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 5526 | 1.11 | 0.000658 |
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Target: 5'- uGGCAGCAAUGCCAAGGCCGUCCUCGAg -3' miRNA: 3'- -CCGUCGUUACGGUUCCGGCAGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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