Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31887 | 5' | -59 | NC_006938.1 | + | 47019 | 0.67 | 0.451635 |
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Target: 5'- cGACGCUGCccuggGCGGCCGaaucuUCGa-GAGGa -3' miRNA: 3'- -UUGCGACGa----UGUCGGCg----AGCggCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 48437 | 0.66 | 0.501 |
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Target: 5'- gGACGC-GCUcgucACcGCCGCccgaGCCGGGGu -3' miRNA: 3'- -UUGCGaCGA----UGuCGGCGag--CGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 49588 | 1.08 | 0.000502 |
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Target: 5'- gAACGCUGCUACAGCCGCUCGCCGAGGg -3' miRNA: 3'- -UUGCGACGAUGUCGGCGAGCGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 50092 | 0.69 | 0.361613 |
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Target: 5'- cAACGCUcagGCcGCGaucGUCGC-CGCCGAGGg -3' miRNA: 3'- -UUGCGA---CGaUGU---CGGCGaGCGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 52734 | 0.7 | 0.33715 |
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Target: 5'- -uCGCUGUUGC-GCCGCUCGaaacccgaCGAGa -3' miRNA: 3'- uuGCGACGAUGuCGGCGAGCg-------GCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 53013 | 0.68 | 0.387283 |
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Target: 5'- uAACGCUug-GCAGCuCGCUCGCCGc-- -3' miRNA: 3'- -UUGCGAcgaUGUCG-GCGAGCGGCucc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 55396 | 0.67 | 0.471074 |
|
Target: 5'- -uUGCaGCaguCGGCgCGCUCGCCGcAGGc -3' miRNA: 3'- uuGCGaCGau-GUCG-GCGAGCGGC-UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 60530 | 0.66 | 0.521409 |
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Target: 5'- --aGCUGCUACaucgcgcauGGCuCGCUCaCCGGGa -3' miRNA: 3'- uugCGACGAUG---------UCG-GCGAGcGGCUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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