Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31887 | 5' | -59 | NC_006938.1 | + | 19793 | 0.7 | 0.336355 |
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Target: 5'- cGCGCUGCUAagugGGaCCgugacuaccucaaGCUCGCCGAGu -3' miRNA: 3'- uUGCGACGAUg---UC-GG-------------CGAGCGGCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 18935 | 0.69 | 0.370037 |
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Target: 5'- cACGCUGCU-CGGCCG-UCGCUGccgcuucagauGGGa -3' miRNA: 3'- uUGCGACGAuGUCGGCgAGCGGC-----------UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 17137 | 0.72 | 0.23917 |
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Target: 5'- --aGCUGCUcgaugaccuCGGCCGCUCGCuuguacuggauCGAGGa -3' miRNA: 3'- uugCGACGAu--------GUCGGCGAGCG-----------GCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 12861 | 0.66 | 0.518327 |
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Target: 5'- cGAC-CUGUgggccaauccuggacCAGgCGCUCGCCGGGGc -3' miRNA: 3'- -UUGcGACGau-------------GUCgGCGAGCGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 9598 | 0.66 | 0.511163 |
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Target: 5'- uACGgUgGgaGgGGCUGCUCGCCGcAGGg -3' miRNA: 3'- uUGCgA-CgaUgUCGGCGAGCGGC-UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 7891 | 0.67 | 0.490928 |
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Target: 5'- --aGCU-CUACGGCUGCUCGCUccuGGu -3' miRNA: 3'- uugCGAcGAUGUCGGCGAGCGGcu-CC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 7807 | 0.77 | 0.101909 |
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Target: 5'- -cUGCUGCUGCAGcCCGUUCaGCCGGGu -3' miRNA: 3'- uuGCGACGAUGUC-GGCGAG-CGGCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 7742 | 0.68 | 0.414125 |
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Target: 5'- uGugGUgGCcggGCAGCCcuuCUCGCUGAGGa -3' miRNA: 3'- -UugCGaCGa--UGUCGGc--GAGCGGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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