Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31887 | 5' | -59 | NC_006938.1 | + | 42507 | 0.69 | 0.373444 |
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Target: 5'- aGGCGUcGCUcCGGCCGCgUCggcgaagaacucgacGCCGAGGa -3' miRNA: 3'- -UUGCGaCGAuGUCGGCG-AG---------------CGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 7742 | 0.68 | 0.414125 |
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Target: 5'- uGugGUgGCcggGCAGCCcuuCUCGCUGAGGa -3' miRNA: 3'- -UugCGaCGa--UGUCGGc--GAGCGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 21555 | 0.67 | 0.442081 |
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Target: 5'- cGACGUUGaaGCAGUgGUcccCGCCGAGGu -3' miRNA: 3'- -UUGCGACgaUGUCGgCGa--GCGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 48437 | 0.66 | 0.501 |
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Target: 5'- gGACGC-GCUcgucACcGCCGCccgaGCCGGGGu -3' miRNA: 3'- -UUGCGaCGA----UGuCGGCGag--CGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 9598 | 0.66 | 0.511163 |
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Target: 5'- uACGgUgGgaGgGGCUGCUCGCCGcAGGg -3' miRNA: 3'- uUGCgA-CgaUgUCGGCGAGCGGC-UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 22025 | 0.66 | 0.521409 |
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Target: 5'- cGCGCUGCaACuGCCGCaccauguaGCCGuuGGa -3' miRNA: 3'- uUGCGACGaUGuCGGCGag------CGGCu-CC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 32791 | 0.66 | 0.531734 |
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Target: 5'- --aGCUGCuUGCGGauCgGCUCGCCGgucucGGGg -3' miRNA: 3'- uugCGACG-AUGUC--GgCGAGCGGC-----UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 7807 | 0.77 | 0.101909 |
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Target: 5'- -cUGCUGCUGCAGcCCGUUCaGCCGGGu -3' miRNA: 3'- uuGCGACGAUGUC-GGCGAG-CGGCUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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