Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31887 | 5' | -59 | NC_006938.1 | + | 18935 | 0.69 | 0.370037 |
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Target: 5'- cACGCUGCU-CGGCCG-UCGCUGccgcuucagauGGGa -3' miRNA: 3'- uUGCGACGAuGUCGGCgAGCGGC-----------UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 53013 | 0.68 | 0.387283 |
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Target: 5'- uAACGCUug-GCAGCuCGCUCGCCGc-- -3' miRNA: 3'- -UUGCGAcgaUGUCG-GCGAGCGGCucc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 47019 | 0.67 | 0.451635 |
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Target: 5'- cGACGCUGCccuggGCGGCCGaaucuUCGa-GAGGa -3' miRNA: 3'- -UUGCGACGa----UGUCGGCg----AGCggCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 29767 | 0.67 | 0.471074 |
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Target: 5'- --aGCaGCUacgacaugGCAGCCauccgGCUCgGCCGAGGg -3' miRNA: 3'- uugCGaCGA--------UGUCGG-----CGAG-CGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 55396 | 0.67 | 0.471074 |
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Target: 5'- -uUGCaGCaguCGGCgCGCUCGCCGcAGGc -3' miRNA: 3'- uuGCGaCGau-GUCG-GCGAGCGGC-UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 7891 | 0.67 | 0.490928 |
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Target: 5'- --aGCU-CUACGGCUGCUCGCUccuGGu -3' miRNA: 3'- uugCGAcGAUGUCGGCGAGCGGcu-CC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 29918 | 0.67 | 0.490928 |
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Target: 5'- -cCGCUGacagACAGCgGUaUCGCgGAGGg -3' miRNA: 3'- uuGCGACga--UGUCGgCG-AGCGgCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 49588 | 1.08 | 0.000502 |
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Target: 5'- gAACGCUGCUACAGCCGCUCGCCGAGGg -3' miRNA: 3'- -UUGCGACGAUGUCGGCGAGCGGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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