Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31887 | 5' | -59 | NC_006938.1 | + | 29918 | 0.67 | 0.490928 |
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Target: 5'- -cCGCUGacagACAGCgGUaUCGCgGAGGg -3' miRNA: 3'- uuGCGACga--UGUCGgCG-AGCGgCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 48437 | 0.66 | 0.501 |
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Target: 5'- gGACGC-GCUcgucACcGCCGCccgaGCCGGGGu -3' miRNA: 3'- -UUGCGaCGA----UGuCGGCGag--CGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 35764 | 0.66 | 0.505055 |
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Target: 5'- cGAC-CUGCUGCAGUCGaUCGUgaccacccgucccuuCGAGGg -3' miRNA: 3'- -UUGcGACGAUGUCGGCgAGCG---------------GCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 9598 | 0.66 | 0.511163 |
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Target: 5'- uACGgUgGgaGgGGCUGCUCGCCGcAGGg -3' miRNA: 3'- uUGCgA-CgaUgUCGGCGAGCGGC-UCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 12861 | 0.66 | 0.518327 |
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Target: 5'- cGAC-CUGUgggccaauccuggacCAGgCGCUCGCCGGGGc -3' miRNA: 3'- -UUGcGACGau-------------GUCgGCGAGCGGCUCC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 22025 | 0.66 | 0.521409 |
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Target: 5'- cGCGCUGCaACuGCCGCaccauguaGCCGuuGGa -3' miRNA: 3'- uUGCGACGaUGuCGGCGag------CGGCu-CC- -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 60530 | 0.66 | 0.521409 |
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Target: 5'- --aGCUGCUACaucgcgcauGGCuCGCUCaCCGGGa -3' miRNA: 3'- uugCGACGAUG---------UCG-GCGAGcGGCUCc -5' |
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| 31887 | 5' | -59 | NC_006938.1 | + | 32791 | 0.66 | 0.531734 |
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Target: 5'- --aGCUGCuUGCGGauCgGCUCGCCGgucucGGGg -3' miRNA: 3'- uugCGACG-AUGUC--GgCGAGCGGC-----UCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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