Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31898 | 5' | -53.9 | NC_006938.1 | + | 1792 | 0.67 | 0.808454 |
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Target: 5'- uCCUGccgaccgccgcGCGACCCCuguccGGGCUUCAGaccuacacCGGCu -3' miRNA: 3'- -GGAC-----------UGCUGGGG-----CCUGAAGUU--------GCUGu -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 61680 | 0.67 | 0.817687 |
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Target: 5'- --cGGCGGCgcggCCCGGACcUCuguGACGACGc -3' miRNA: 3'- ggaCUGCUG----GGGCCUGaAG---UUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 22416 | 0.67 | 0.817687 |
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Target: 5'- gCgaGACGGCCCUGG---UCAugcGCGGCAa -3' miRNA: 3'- -GgaCUGCUGGGGCCugaAGU---UGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 62281 | 0.66 | 0.835578 |
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Target: 5'- cCCUG-C--UCCUGGGCUUCGACGcGCAg -3' miRNA: 3'- -GGACuGcuGGGGCCUGAAGUUGC-UGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 26030 | 0.66 | 0.835578 |
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Target: 5'- -aUGGCGGCCUCGuccaGCUUgcCAGCGGCGg -3' miRNA: 3'- ggACUGCUGGGGCc---UGAA--GUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 12083 | 0.66 | 0.835578 |
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Target: 5'- gCCUcGACuGCCCCGG-CUcCGgccGCGACGa -3' miRNA: 3'- -GGA-CUGcUGGGGCCuGAaGU---UGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 58039 | 0.66 | 0.85264 |
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Target: 5'- --gGACGGCCuuGGcauuGCUgcCAGCGGCGg -3' miRNA: 3'- ggaCUGCUGGggCC----UGAa-GUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 20303 | 0.66 | 0.868807 |
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Target: 5'- --gGACGgcACUCCGGACgUCAACcACAu -3' miRNA: 3'- ggaCUGC--UGGGGCCUGaAGUUGcUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 58594 | 0.66 | 0.868807 |
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Target: 5'- gCCUGGgcacUGuccCCCUGGGCga-GACGACAg -3' miRNA: 3'- -GGACU----GCu--GGGGCCUGaagUUGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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