Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31898 | 5' | -53.9 | NC_006938.1 | + | 53386 | 0.67 | 0.799041 |
|
Target: 5'- aCCUgGACG-UCCUGGGCUUCcugGACAg -3' miRNA: 3'- -GGA-CUGCuGGGGCCUGAAGuugCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 54444 | 1.11 | 0.00149 |
|
Target: 5'- cCCUGACGACCCCGGACUUCAACGACAg -3' miRNA: 3'- -GGACUGCUGGGGCCUGAAGUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 56434 | 0.72 | 0.505074 |
|
Target: 5'- --cGACGACCuuGGGCUccUCGACGcggACAg -3' miRNA: 3'- ggaCUGCUGGggCCUGA--AGUUGC---UGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 58039 | 0.66 | 0.85264 |
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Target: 5'- --gGACGGCCuuGGcauuGCUgcCAGCGGCGg -3' miRNA: 3'- ggaCUGCUGGggCC----UGAa-GUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 58594 | 0.66 | 0.868807 |
|
Target: 5'- gCCUGGgcacUGuccCCCUGGGCga-GACGACAg -3' miRNA: 3'- -GGACU----GCu--GGGGCCUGaagUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 60993 | 0.69 | 0.665169 |
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Target: 5'- gCCUGGaGACCCCGGcguGCUgCAGgGACc -3' miRNA: 3'- -GGACUgCUGGGGCC---UGAaGUUgCUGu -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 61680 | 0.67 | 0.817687 |
|
Target: 5'- --cGGCGGCgcggCCCGGACcUCuguGACGACGc -3' miRNA: 3'- ggaCUGCUG----GGGCCUGaAG---UUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 61917 | 0.67 | 0.779718 |
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Target: 5'- aCC-GACGgcACCCUGGGCUUCuuuacguuUGACAc -3' miRNA: 3'- -GGaCUGC--UGGGGCCUGAAGuu------GCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 62281 | 0.66 | 0.835578 |
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Target: 5'- cCCUG-C--UCCUGGGCUUCGACGcGCAg -3' miRNA: 3'- -GGACuGcuGGGGCCUGAAGUUGC-UGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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