Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31898 | 5' | -53.9 | NC_006938.1 | + | 60993 | 0.69 | 0.665169 |
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Target: 5'- gCCUGGaGACCCCGGcguGCUgCAGgGACc -3' miRNA: 3'- -GGACUgCUGGGGCC---UGAaGUUgCUGu -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 50752 | 0.7 | 0.654364 |
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Target: 5'- gCCUGGCG-UCCgGGGCacugcgCGGCGACGa -3' miRNA: 3'- -GGACUGCuGGGgCCUGaa----GUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 27369 | 0.7 | 0.632705 |
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Target: 5'- -aUGGCGuCCCCGucGAUggCAGCGACAc -3' miRNA: 3'- ggACUGCuGGGGC--CUGaaGUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 37599 | 0.7 | 0.632705 |
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Target: 5'- cCCaGACGGCCCC--GCUggAGCGACAg -3' miRNA: 3'- -GGaCUGCUGGGGccUGAagUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 19635 | 0.72 | 0.512274 |
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Target: 5'- aCCagGACGGCCUCcagcagaaggccaaGGACUUCGGCGAg- -3' miRNA: 3'- -GGa-CUGCUGGGG--------------CCUGAAGUUGCUgu -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 56434 | 0.72 | 0.505074 |
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Target: 5'- --cGACGACCuuGGGCUccUCGACGcggACAg -3' miRNA: 3'- ggaCUGCUGGggCCUGA--AGUUGC---UGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 23192 | 0.75 | 0.367135 |
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Target: 5'- aCCUGcCGACCCUGGaggagugcagggaccAgUUCGACGACGu -3' miRNA: 3'- -GGACuGCUGGGGCC---------------UgAAGUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 47819 | 0.85 | 0.090529 |
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Target: 5'- gUCUGgcGCGACCCCGGACgcggacaUCAGCGGCAg -3' miRNA: 3'- -GGAC--UGCUGGGGCCUGa------AGUUGCUGU- -5' |
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| 31898 | 5' | -53.9 | NC_006938.1 | + | 54444 | 1.11 | 0.00149 |
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Target: 5'- cCCUGACGACCCCGGACUUCAACGACAg -3' miRNA: 3'- -GGACUGCUGGGGCCUGAAGUUGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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