Results 1 - 20 of 71 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32568 | 3' | -45.7 | NC_007151.1 | + | 28892 | 0.66 | 0.999993 |
Target: 5'- -aUCGUCGauuggaacgaUgGAAAUCGUCAUggCGACGa -3' miRNA: 3'- ucAGCAGU----------AgCUUUAGUAGUA--GCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 107666 | 0.66 | 0.999993 |
Target: 5'- cGGUCGUUuuaAUCGcgGcCGUUGUCGGCGa -3' miRNA: 3'- -UCAGCAG---UAGCuuUaGUAGUAGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 134551 | 0.66 | 0.999993 |
Target: 5'- cGUUGUCAUCGAuAUUaauguauagauaGUUGUCGAUc -3' miRNA: 3'- uCAGCAGUAGCUuUAG------------UAGUAGCUGu -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 12038 | 0.66 | 0.999993 |
Target: 5'- uGUUGUgGUC--AGUCAUCAUCGGg- -3' miRNA: 3'- uCAGCAgUAGcuUUAGUAGUAGCUgu -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 37877 | 0.66 | 0.99999 |
Target: 5'- cGUUcgGUCAcCGAAGUCAUCAgCGAa- -3' miRNA: 3'- uCAG--CAGUaGCUUUAGUAGUaGCUgu -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 140551 | 0.66 | 0.999986 |
Target: 5'- cGUCG-CcgCGGGAcgAUCGUCGGCGu -3' miRNA: 3'- uCAGCaGuaGCUUUagUAGUAGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 11731 | 0.66 | 0.999986 |
Target: 5'- uGUUGUCGUgGAAuacgauuggGUCGUCGaUGACAu -3' miRNA: 3'- uCAGCAGUAgCUU---------UAGUAGUaGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 132869 | 0.66 | 0.999986 |
Target: 5'- uGGcCG-CAUCGAuGUCGUCAaguccgaugccuUCGGCGa -3' miRNA: 3'- -UCaGCaGUAGCUuUAGUAGU------------AGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 90006 | 0.66 | 0.999986 |
Target: 5'- uGUUGUCAaCGAuuUCGUCAU-GAUAa -3' miRNA: 3'- uCAGCAGUaGCUuuAGUAGUAgCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 44504 | 0.66 | 0.999981 |
Target: 5'- -uUCGUCGUCG---UCGUCGcuuUCGACc -3' miRNA: 3'- ucAGCAGUAGCuuuAGUAGU---AGCUGu -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 74681 | 0.66 | 0.999981 |
Target: 5'- ----aUCAUCGAGAUUacgAUCGUCGAUg -3' miRNA: 3'- ucagcAGUAGCUUUAG---UAGUAGCUGu -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 143082 | 0.66 | 0.999977 |
Target: 5'- gAGUCGUCGggaUCaacgacggacgauuuGGAcUCAUCAUCGAUu -3' miRNA: 3'- -UCAGCAGU---AG---------------CUUuAGUAGUAGCUGu -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 109464 | 0.67 | 0.999964 |
Target: 5'- uGUCGUCAgCGAcuauggCGUCGACGa -3' miRNA: 3'- uCAGCAGUaGCUuuaguaGUAGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 18538 | 0.67 | 0.999959 |
Target: 5'- cGUCGcCAUCGGuuUCGUCGaagagcacucgagCGACAc -3' miRNA: 3'- uCAGCaGUAGCUuuAGUAGUa------------GCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 123109 | 0.67 | 0.999951 |
Target: 5'- cGUCGUCGacguaaaucUCGAuGUCGaUGUCGGCGa -3' miRNA: 3'- uCAGCAGU---------AGCUuUAGUaGUAGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 37554 | 0.67 | 0.999951 |
Target: 5'- -aUCGUCAUCGuc-UCgaaaacuacuGUCGUCGACu -3' miRNA: 3'- ucAGCAGUAGCuuuAG----------UAGUAGCUGu -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 89877 | 0.67 | 0.999935 |
Target: 5'- --cCGUgAUCGAucagAAUCGUCGaCGACAa -3' miRNA: 3'- ucaGCAgUAGCU----UUAGUAGUaGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 78266 | 0.67 | 0.999935 |
Target: 5'- -uUCGg-AUUGAAcguuAUCGUCAUCGACGa -3' miRNA: 3'- ucAGCagUAGCUU----UAGUAGUAGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 101541 | 0.67 | 0.999923 |
Target: 5'- uGUCGUCAcCGAcaaugucgacaauaaAAUUGUCAaCGACAg -3' miRNA: 3'- uCAGCAGUaGCU---------------UUAGUAGUaGCUGU- -5' |
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32568 | 3' | -45.7 | NC_007151.1 | + | 37156 | 0.67 | 0.999906 |
Target: 5'- -uUUGUCGUCGAcgccaaaagacgaaAAUUuaauGUCAUCGACGa -3' miRNA: 3'- ucAGCAGUAGCU--------------UUAG----UAGUAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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