Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 356 | 5' | -58.1 | AC_000011.1 | + | 8206 | 0.68 | 0.294954 |
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Target: 5'- ---cGCAGGCCGGagcuGUCCAGGGuCCUGa -3' miRNA: 3'- agguCGUUCGGCC----CGGGUUUCuGGAC- -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 11895 | 0.69 | 0.287488 |
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Target: 5'- cCCAGCc-GCCGGGCCUuGGAGGCg-- -3' miRNA: 3'- aGGUCGuuCGGCCCGGG-UUUCUGgac -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 9433 | 0.69 | 0.28675 |
|
Target: 5'- cUCCAGguGGCCGGGgggguCCCcguugggcagggaGAGGGCgCUGa -3' miRNA: 3'- -AGGUCguUCGGCCC-----GGG-------------UUUCUG-GAC- -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 33851 | 0.71 | 0.182469 |
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Target: 5'- cUCAGgGGGuCCGGGUCCAGGGACUc- -3' miRNA: 3'- aGGUCgUUC-GGCCCGGGUUUCUGGac -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 21127 | 0.71 | 0.182469 |
|
Target: 5'- cUCCGGCGAGCaGGaGCUCAGGgccaucauccgcGACCUGg -3' miRNA: 3'- -AGGUCGUUCGgCC-CGGGUUU------------CUGGAC- -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 19188 | 0.75 | 0.109646 |
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Target: 5'- gUCAGCAAGCCauGCCCAAcAGACCUa -3' miRNA: 3'- aGGUCGUUCGGccCGGGUU-UCUGGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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