Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 356 | 5' | -58.1 | AC_000011.1 | + | 5151 | 0.68 | 0.310337 |
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Target: 5'- gUCCAGCGcAGCCaGGGUCCGGu--CCUu -3' miRNA: 3'- -AGGUCGU-UCGG-CCCGGGUUucuGGAc -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 26514 | 0.67 | 0.334541 |
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Target: 5'- cUCAGC--GCCGGGCCCGcgauGAUCUc -3' miRNA: 3'- aGGUCGuuCGGCCCGGGUuu--CUGGAc -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 11406 | 0.67 | 0.342911 |
|
Target: 5'- aCCAGCAAGCCGcugacGGCgCAGcuguuCCUGg -3' miRNA: 3'- aGGUCGUUCGGC-----CCGgGUUucu--GGAC- -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 6270 | 0.67 | 0.360098 |
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Target: 5'- gUCAGCucGUCGGGCaCGAuucuGACCUGc -3' miRNA: 3'- aGGUCGuuCGGCCCGgGUUu---CUGGAC- -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 8479 | 0.66 | 0.386987 |
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Target: 5'- -gCGGCAggggcGGCUcgGGGCCCGGAGGCa-- -3' miRNA: 3'- agGUCGU-----UCGG--CCCGGGUUUCUGgac -5' |
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| 356 | 5' | -58.1 | AC_000011.1 | + | 21970 | 0.82 | 0.029759 |
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Target: 5'- cCCAGCAGGUCGGGCgCGGAGAUCUu -3' miRNA: 3'- aGGUCGUUCGGCCCGgGUUUCUGGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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