Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 360 | 5' | -62.5 | AC_000011.1 | + | 15881 | 0.67 | 0.223827 |
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Target: 5'- gGCCaGACGcGC-GGCUUcagGCgccAGCGCCg -3' miRNA: 3'- aCGGcCUGC-CGaCCGAGa--CG---UCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 15927 | 0.67 | 0.206924 |
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Target: 5'- cGCgCGGccACGGC-GGCg--GCAGCgGCCa -3' miRNA: 3'- aCG-GCC--UGCCGaCCGagaCGUCG-CGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 17354 | 0.68 | 0.196278 |
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Target: 5'- -uCCGuGGCGGUgcaGGcCUCU-CAGCGCCa -3' miRNA: 3'- acGGC-CUGCCGa--CC-GAGAcGUCGCGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 10143 | 0.69 | 0.162711 |
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Target: 5'- gUGCgGcGGCGGCUGGCg--GUAGagcgGCCa -3' miRNA: 3'- -ACGgC-CUGCCGACCGagaCGUCg---CGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 3585 | 0.69 | 0.15836 |
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Target: 5'- cGgUGGACGGCcGGCcCgUGCAGC-CCg -3' miRNA: 3'- aCgGCCUGCCGaCCGaG-ACGUCGcGG- -5' |
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| 360 | 5' | -62.5 | AC_000011.1 | + | 8409 | 0.7 | 0.127184 |
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Target: 5'- -cUUGGGCGGCUGGggCgacggggGCGGUGCCu -3' miRNA: 3'- acGGCCUGCCGACCgaGa------CGUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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