Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 408 | 5' | -62.7 | AC_000011.1 | + | 11494 | 0.71 | 0.114103 |
|
Target: 5'- cCGAGCCcgagGGCCGCuGGCUCCuggaccuGGUGa -3' miRNA: 3'- cGUUCGGua--CCGGCGcCCGAGG-------CCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 11232 | 0.69 | 0.150063 |
|
Target: 5'- cGCGcGCaCGUGGCCGCGGccaaccuGgUCaCGGCGu -3' miRNA: 3'- -CGUuCG-GUACCGGCGCC-------CgAG-GCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 8242 | 0.67 | 0.22394 |
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Target: 5'- -gGAGUCA-GGUCaGUGGGCagCGGCGg -3' miRNA: 3'- cgUUCGGUaCCGG-CGCCCGagGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 5858 | 0.69 | 0.167594 |
|
Target: 5'- uGUAGGCCAcgUGaCCGgGGGUcCCGGCc -3' miRNA: 3'- -CGUUCGGU--ACcGGCgCCCGaGGCCGc -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 5349 | 0.66 | 0.241882 |
|
Target: 5'- ---cGCCucGGCCGCGuGGCcUuuGGCGc -3' miRNA: 3'- cguuCGGuaCCGGCGC-CCG-AggCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 3704 | 0.71 | 0.117626 |
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Target: 5'- cGCGgaauGGCCAUGGgCGCcGGCUacuaCGGCa -3' miRNA: 3'- -CGU----UCGGUACCgGCGcCCGAg---GCCGc -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 3230 | 0.66 | 0.274429 |
|
Target: 5'- uCAGGCucacucuggaCAUGGCauCGGGCUCCaGCa -3' miRNA: 3'- cGUUCG----------GUACCGgcGCCCGAGGcCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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