Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 408 | 5' | -62.7 | AC_000011.1 | + | 5858 | 0.69 | 0.167594 |
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Target: 5'- uGUAGGCCAcgUGaCCGgGGGUcCCGGCc -3' miRNA: 3'- -CGUUCGGU--ACcGGCgCCCGaGGCCGc -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 35746 | 0.68 | 0.172141 |
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Target: 5'- cCAGGCa--GGCCaCGGGguCUCCGGCGc -3' miRNA: 3'- cGUUCGguaCCGGcGCCC--GAGGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 13926 | 0.67 | 0.207141 |
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Target: 5'- aGCAGGCgAUGGCgGCGG----CGGCGa -3' miRNA: 3'- -CGUUCGgUACCGgCGCCcgagGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 16712 | 0.67 | 0.207141 |
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Target: 5'- uCAAGCagGUGGCCcCGGGCcUgGGCGu -3' miRNA: 3'- cGUUCGg-UACCGGcGCCCGaGgCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 8242 | 0.67 | 0.22394 |
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Target: 5'- -gGAGUCA-GGUCaGUGGGCagCGGCGg -3' miRNA: 3'- cgUUCGGUaCCGG-CGCCCGagGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 12379 | 0.66 | 0.235772 |
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Target: 5'- cGCgAGGCCGUGGCCcagcGCGagcGGUUCCaccGCGa -3' miRNA: 3'- -CG-UUCGGUACCGG----CGC---CCGAGGc--CGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 21108 | 1.1 | 0.000093 |
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Target: 5'- uGCAAGCCAUGGCCGCGGGCUCCGGCGa -3' miRNA: 3'- -CGUUCGGUACCGGCGCCCGAGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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