Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5129 | 5' | -56.1 | NC_001798.1 | + | 22286 | 0.69 | 0.745165 |
Target: 5'- aUGCCGCGCGGgCggaGCGGCgGCggCGc -3' miRNA: 3'- gACGGCGCGCC-Ga--UGCUGaUGaaGCa -5' |
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5129 | 5' | -56.1 | NC_001798.1 | + | 5994 | 0.67 | 0.851616 |
Target: 5'- -gGCUGCGCGGCggagaccgggACGGCaGCggCGg -3' miRNA: 3'- gaCGGCGCGCCGa---------UGCUGaUGaaGCa -5' |
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5129 | 5' | -56.1 | NC_001798.1 | + | 4840 | 0.77 | 0.339066 |
Target: 5'- -gGCCGCGCGGCgggGCGACgguccgGgUUCGg -3' miRNA: 3'- gaCGGCGCGCCGa--UGCUGa-----UgAAGCa -5' |
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5129 | 5' | -56.1 | NC_001798.1 | + | 3304 | 0.66 | 0.900839 |
Target: 5'- -cGCCGCcgacggcaacgggGCGGCggcgGCGGCggGCUUCc- -3' miRNA: 3'- gaCGGCG-------------CGCCGa---UGCUGa-UGAAGca -5' |
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5129 | 5' | -56.1 | NC_001798.1 | + | 2570 | 0.68 | 0.832189 |
Target: 5'- -gGCCGCGgGGCggggggcguccgcGCGGCUcuuCUUCGg -3' miRNA: 3'- gaCGGCGCgCCGa------------UGCUGAu--GAAGCa -5' |
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5129 | 5' | -56.1 | NC_001798.1 | + | 2501 | 0.71 | 0.655185 |
Target: 5'- -cGCCGCGgGGCgcgGCGGCcGCggCGg -3' miRNA: 3'- gaCGGCGCgCCGa--UGCUGaUGaaGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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