Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5143 | 5' | -56.3 | NC_001798.1 | + | 1253 | 0.67 | 0.817838 |
Target: 5'- gGGGCg-GGCcCGCGUCC-GCGUcgucGCGCa -3' miRNA: 3'- -UCCGgaCCGaGCGCAGGaUGUA----UGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 11986 | 0.66 | 0.866619 |
Target: 5'- gGGGCUgugGGCguggUGCGUCgaGCG-ACACa -3' miRNA: 3'- -UCCGGa--CCGa---GCGCAGgaUGUaUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 20462 | 0.74 | 0.462668 |
Target: 5'- uGGGCCUGGCgagcagaGCG-CCggugcGCGUGCGCg -3' miRNA: 3'- -UCCGGACCGag-----CGCaGGa----UGUAUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 24769 | 0.71 | 0.601253 |
Target: 5'- -cGCCUGGCUgCGCGagCUGCgguucGUGCGCg -3' miRNA: 3'- ucCGGACCGA-GCGCagGAUG-----UAUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 25496 | 0.66 | 0.881239 |
Target: 5'- cGGGCCcgcUGcGCcgCGCGgcggCCUGgAUGCGCc -3' miRNA: 3'- -UCCGG---AC-CGa-GCGCa---GGAUgUAUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 25965 | 0.67 | 0.851145 |
Target: 5'- uGGCC-GGCggCGCGggaCCUGCGccGCACc -3' miRNA: 3'- uCCGGaCCGa-GCGCa--GGAUGUa-UGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 31762 | 0.67 | 0.843102 |
Target: 5'- uGGGCCgGGCaggCGCGaCCgACGcGCGCg -3' miRNA: 3'- -UCCGGaCCGa--GCGCaGGaUGUaUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 32242 | 0.68 | 0.780869 |
Target: 5'- cGGGgCUGGUgggagcgUCGCGUCC-GCGUccgGCGCu -3' miRNA: 3'- -UCCgGACCG-------AGCGCAGGaUGUA---UGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 39476 | 0.7 | 0.693668 |
Target: 5'- gGGGCCUGGCgCGUG-CCU-CGUGgcCGCg -3' miRNA: 3'- -UCCGGACCGaGCGCaGGAuGUAU--GUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 40409 | 0.7 | 0.693668 |
Target: 5'- cAGGUCUGGgUC-CG-CCUucGCGUACACg -3' miRNA: 3'- -UCCGGACCgAGcGCaGGA--UGUAUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 43126 | 0.69 | 0.713857 |
Target: 5'- cGGCg-GGCgCGCGUCCcGCGUcACGCg -3' miRNA: 3'- uCCGgaCCGaGCGCAGGaUGUA-UGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 43226 | 0.71 | 0.590996 |
Target: 5'- gGGGUUUGGaccgaCGCGUCCUGCAUGagaGCc -3' miRNA: 3'- -UCCGGACCga---GCGCAGGAUGUAUg--UG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 49586 | 0.72 | 0.580768 |
Target: 5'- -cGCCUGGCgCGCGUCCUguuucuGCAucUAUACc -3' miRNA: 3'- ucCGGACCGaGCGCAGGA------UGU--AUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 52171 | 0.68 | 0.781801 |
Target: 5'- gAGGCCgcguuugcGGggCGCGUCCUggACGUGCugGCg -3' miRNA: 3'- -UCCGGa-------CCgaGCGCAGGA--UGUAUG--UG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 53245 | 0.67 | 0.817838 |
Target: 5'- cGGCCgUGG---GCGUCCUGCGgcaGCGCg -3' miRNA: 3'- uCCGG-ACCgagCGCAGGAUGUa--UGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 59561 | 0.67 | 0.851145 |
Target: 5'- gGGGCUggGGCUCGCGUCgccgguCUGgAgguCGCg -3' miRNA: 3'- -UCCGGa-CCGAGCGCAG------GAUgUau-GUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 60779 | 0.7 | 0.659934 |
Target: 5'- aAGGCCUGcaggaucccguucaGCUCgGCGcCCUGCA-GCACu -3' miRNA: 3'- -UCCGGAC--------------CGAG-CGCaGGAUGUaUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 61917 | 0.73 | 0.485385 |
Target: 5'- -cGCCUGGCUcaccuguucuguaucCGCGUUCUGCGgGCGCu -3' miRNA: 3'- ucCGGACCGA---------------GCGCAGGAUGUaUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 65762 | 0.76 | 0.37461 |
Target: 5'- cGGuGCCggGGCUgGCGUcguugaacgCCUGCAUGCACa -3' miRNA: 3'- -UC-CGGa-CCGAgCGCA---------GGAUGUAUGUG- -5' |
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5143 | 5' | -56.3 | NC_001798.1 | + | 71603 | 0.66 | 0.888214 |
Target: 5'- cGGCCUgucgGGCg-GCGUCCU-CAgccagACGCu -3' miRNA: 3'- uCCGGA----CCGagCGCAGGAuGUa----UGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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