Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5151 | 3' | -62.1 | NC_001798.1 | + | 36396 | 0.66 | 0.645074 |
Target: 5'- gCGCGGcGCCgggGCCCCCCugccgggcggggcGGUGGg--- -3' miRNA: 3'- aGCGCC-CGGa--CGGGGGG-------------CUACUagag -5' |
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5151 | 3' | -62.1 | NC_001798.1 | + | 59799 | 0.66 | 0.646057 |
Target: 5'- aCcCGGGCCUGgCCCUCGAggccuUCUUc -3' miRNA: 3'- aGcGCCCGGACgGGGGGCUacu--AGAG- -5' |
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5151 | 3' | -62.1 | NC_001798.1 | + | 46410 | 0.66 | 0.646057 |
Target: 5'- -gGCaGGGCCaccGcCCCCCCGgcGGUCg- -3' miRNA: 3'- agCG-CCCGGa--C-GGGGGGCuaCUAGag -5' |
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5151 | 3' | -62.1 | NC_001798.1 | + | 74631 | 0.66 | 0.65392 |
Target: 5'- cUG-GGGCCgaccgGCCCCCCGAggccccccggggGGcCUCg -3' miRNA: 3'- aGCgCCCGGa----CGGGGGGCUa-----------CUaGAG- -5' |
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5151 | 3' | -62.1 | NC_001798.1 | + | 76608 | 0.66 | 0.665696 |
Target: 5'- -gGCGGG-CUGUCCCCCGAg------ -3' miRNA: 3'- agCGCCCgGACGGGGGGCUacuagag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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