miRNA: 3'- aGCgCCCGGACgGGGgGCUACuaGag -5'
miRNA: 3'- aGCGCC-CGGaCGG-GGGGCuacuaGAG- -5'
miRNA: 3'- aGCG--CCCGGa---CGGGGGgCuaCUAGag -5'
miRNA: 3'- -AGCGCCC------GGACGGGGGGCuaC-------UAGAG- -5'
miRNA: 3'- aGCGCCCgGACGG-GGGGCuaCUagag -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5151 | 3' | -62.1 | NC_001798.1 | + | 134985 | 0.68 | 0.51979 |
|
Target: 5'- cUGgGGGCCUGgCCCgCGGUGggCg- -3' miRNA: 3'- aGCgCCCGGACgGGGgGCUACuaGag -5' |
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| 5151 | 3' | -62.1 | NC_001798.1 | + | 138404 | 0.66 | 0.636219 |
|
Target: 5'- gCGCGGcGCCaGCCaCCCCGcg---CUCg -3' miRNA: 3'- aGCGCC-CGGaCGG-GGGGCuacuaGAG- -5' |
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| 5151 | 3' | -62.1 | NC_001798.1 | + | 143294 | 0.68 | 0.491841 |
|
Target: 5'- aCGUagGGGCCguccGCCCCCgGcgGGUCg- -3' miRNA: 3'- aGCG--CCCGGa---CGGGGGgCuaCUAGag -5' |
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| 5151 | 3' | -62.1 | NC_001798.1 | + | 150649 | 0.68 | 0.528298 |
|
Target: 5'- cUCGCGGGgggcguCCUGCCCUCCGccGccgcggcGUCUUc -3' miRNA: 3'- -AGCGCCC------GGACGGGGGGCuaC-------UAGAG- -5' |
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| 5151 | 3' | -62.1 | NC_001798.1 | + | 154516 | 0.67 | 0.58712 |
|
Target: 5'- cCGCGGGgCUGCCuUCCCGcgGGc--- -3' miRNA: 3'- aGCGCCCgGACGG-GGGGCuaCUagag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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