Results 1 - 20 of 105 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5549 | 3' | -62.4 | NC_001806.1 | + | 24003 | 0.66 | 0.676229 |
Target: 5'- uGGCgGCccUGGCCaaCCGgcugUgCGGGCCg -3' miRNA: 3'- -CCG-CGacACCGGagGGCa---AgGCCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 116534 | 0.66 | 0.676229 |
Target: 5'- uGGCGCgcgaaaucGUGGCgCggCUCGUggccaggggcaUCCGGGaCCu -3' miRNA: 3'- -CCGCGa-------CACCG-Ga-GGGCA-----------AGGCCC-GG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 108543 | 0.66 | 0.676229 |
Target: 5'- cGCGUgcgGcUGGCCgcgCCCGccgcUCCGcGGUCa -3' miRNA: 3'- cCGCGa--C-ACCGGa--GGGCa---AGGC-CCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 23717 | 0.66 | 0.676229 |
Target: 5'- cGCGCgGUGcGCCgggCCCGcccccgCCGcccaGGCCg -3' miRNA: 3'- cCGCGaCAC-CGGa--GGGCaa----GGC----CCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 72534 | 0.66 | 0.673332 |
Target: 5'- cGGCGUg--GGCC-CCCGggaggggguagggggCCcgGGGCCa -3' miRNA: 3'- -CCGCGacaCCGGaGGGCaa-------------GG--CCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 3969 | 0.66 | 0.670432 |
Target: 5'- cGCGCggGUucuggagccaccccaGGCCUCCgCG-UCCGGGgUg -3' miRNA: 3'- cCGCGa-CA---------------CCGGAGG-GCaAGGCCCgG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 37765 | 0.66 | 0.666561 |
Target: 5'- cGGCgGCgg-GcGCCUUUCGcUCCGgGGCCg -3' miRNA: 3'- -CCG-CGacaC-CGGAGGGCaAGGC-CCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 134316 | 0.66 | 0.666561 |
Target: 5'- gGGCggGUUGuUGGCCagCagGUaCCGGGCCa -3' miRNA: 3'- -CCG--CGAC-ACCGGagGg-CAaGGCCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 35823 | 0.66 | 0.656868 |
Target: 5'- gGGCGCgugGcGGCCUCCUa----GGGUCa -3' miRNA: 3'- -CCGCGa--CaCCGGAGGGcaaggCCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 11259 | 0.66 | 0.656868 |
Target: 5'- cGGUuaUGUGGUgCUCCCGg-CCaGuGGCCg -3' miRNA: 3'- -CCGcgACACCG-GAGGGCaaGG-C-CCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 42379 | 0.66 | 0.656868 |
Target: 5'- aGGuCGUacGUGGCC-CCCGcgCCccccagacgcuGGGCCu -3' miRNA: 3'- -CC-GCGa-CACCGGaGGGCaaGG-----------CCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 119903 | 0.66 | 0.647157 |
Target: 5'- cGGCGC--UGGCCacggCCCugaCCGaGGCCu -3' miRNA: 3'- -CCGCGacACCGGa---GGGcaaGGC-CCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 32982 | 0.66 | 0.647157 |
Target: 5'- -cCGCcG-GGgC-CCCGggCCGGGCCg -3' miRNA: 3'- ccGCGaCaCCgGaGGGCaaGGCCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 1646 | 0.66 | 0.644241 |
Target: 5'- cGGCGUgcgaGUgGGCCUCcuccucgcagaaguCCGgcgcgCCGGGCg -3' miRNA: 3'- -CCGCGa---CA-CCGGAG--------------GGCaa---GGCCCGg -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 57833 | 0.66 | 0.637435 |
Target: 5'- gGGUGCgGUGGgCgacCCCGUcuuagguuUCUuuaGGGCCg -3' miRNA: 3'- -CCGCGaCACCgGa--GGGCA--------AGG---CCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 135596 | 0.66 | 0.637435 |
Target: 5'- cGCGCUGcUGGCgCUCCa-----GGGCCc -3' miRNA: 3'- cCGCGAC-ACCG-GAGGgcaaggCCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 30177 | 0.66 | 0.637435 |
Target: 5'- -cCGCcccgGGCCcCCCGcgUCCGcGGCCg -3' miRNA: 3'- ccGCGaca-CCGGaGGGCa-AGGC-CCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 3351 | 0.66 | 0.637435 |
Target: 5'- gGGgGCUGgcgGGCCgggcCCCGgccagccCCGGGaCg -3' miRNA: 3'- -CCgCGACa--CCGGa---GGGCaa-----GGCCCgG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 2675 | 0.66 | 0.637435 |
Target: 5'- gGGCGCcgccgUGUGGCUgggcCCCGg---GGGCUg -3' miRNA: 3'- -CCGCG-----ACACCGGa---GGGCaaggCCCGG- -5' |
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5549 | 3' | -62.4 | NC_001806.1 | + | 150680 | 0.66 | 0.627709 |
Target: 5'- cGCGCUGgcggccgccgaUGGCCagUCCCGcccCCGaGGCg -3' miRNA: 3'- cCGCGAC-----------ACCGG--AGGGCaa-GGC-CCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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