Results 1 - 20 of 384 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5550 | 3' | -60.8 | NC_001806.1 | + | 138734 | 0.66 | 0.694303 |
Target: 5'- gGCGGAGauucugucucuGCGCCCcaaaucuucgGGGGUGCCGacgcaGGCg -3' miRNA: 3'- -CGCUUU-----------CGCGGG----------CCUCGCGGUg----CCGg -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 94151 | 0.66 | 0.703003 |
Target: 5'- aGCuGAAcGCGCUucaggugCGG-GCGCUGCGGCg -3' miRNA: 3'- -CG-CUUuCGCGG-------GCCuCGCGGUGCCGg -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 65444 | 0.66 | 0.694303 |
Target: 5'- -aGGGAGCG---GGGGaUGCCGCGGCCc -3' miRNA: 3'- cgCUUUCGCgggCCUC-GCGGUGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 113052 | 0.66 | 0.703967 |
Target: 5'- cGCGAccccgucgAAGcCGCCaauccguaCGGGGCGUacgugGCGGCCc -3' miRNA: 3'- -CGCU--------UUC-GCGG--------GCCUCGCGg----UGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 91344 | 0.66 | 0.703967 |
Target: 5'- -----cGUGCCCGGGacccaCGUgGCGGCCa -3' miRNA: 3'- cgcuuuCGCGGGCCUc----GCGgUGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 74560 | 0.66 | 0.689453 |
Target: 5'- cGCGGccuuuguuccuGCGCCCGGcGCGUCgcccuaccccgacgACGGCg -3' miRNA: 3'- -CGCUuu---------CGCGGGCCuCGCGG--------------UGCCGg -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 26702 | 0.66 | 0.703967 |
Target: 5'- gGCGu--GgGCCCGGGGgGCgG-GGCUg -3' miRNA: 3'- -CGCuuuCgCGGGCCUCgCGgUgCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 2813 | 0.66 | 0.703967 |
Target: 5'- gGCGggGGCGggcucgggcCCCgGGGGCGUggaggggggCGCGGgCg -3' miRNA: 3'- -CGCuuUCGC---------GGG-CCUCGCG---------GUGCCgG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 27827 | 0.66 | 0.698175 |
Target: 5'- uUGggGGCGCCgGGuugguccccggggacGGgGCCGCcccgcggugGGCCu -3' miRNA: 3'- cGCuuUCGCGGgCC---------------UCgCGGUG---------CCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 120865 | 0.66 | 0.703967 |
Target: 5'- gGCGGccccuGGCGCcgccuggucccCCGGGGaccuuGCCcCGGCCg -3' miRNA: 3'- -CGCUu----UCGCG-----------GGCCUCg----CGGuGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 26834 | 0.66 | 0.694303 |
Target: 5'- gGgGAGGGgGCgCCGGAGCcccCCGCGaCCc -3' miRNA: 3'- -CgCUUUCgCG-GGCCUCGc--GGUGCcGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 127899 | 0.66 | 0.703967 |
Target: 5'- gGCGcgGGUGCagCGGAGC---ACGGCCa -3' miRNA: 3'- -CGCuuUCGCGg-GCCUCGcggUGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 78688 | 0.66 | 0.694303 |
Target: 5'- cUGGgcGCGCCCGucgucguGGCGCuccgcaacacCACGGCCu -3' miRNA: 3'- cGCUuuCGCGGGCc------UCGCG----------GUGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 139332 | 0.66 | 0.694303 |
Target: 5'- aGCGAGuuuGUcgaGUCCauugacucuacGGAGCugGCCAUGGCCa -3' miRNA: 3'- -CGCUUu--CG---CGGG-----------CCUCG--CGGUGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 136338 | 0.66 | 0.703003 |
Target: 5'- uCGAAggccAGCGCcagcucaaaaaacCCGGGGCcggcgcgcuCCGCGGCCu -3' miRNA: 3'- cGCUU----UCGCG-------------GGCCUCGc--------GGUGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 26640 | 0.66 | 0.694303 |
Target: 5'- cCGAGccGGCGCCa-GAG-GCC-CGGCCc -3' miRNA: 3'- cGCUU--UCGCGGgcCUCgCGGuGCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 132686 | 0.66 | 0.694303 |
Target: 5'- cUGggGGCcugGCCCGcgguGGGgGCCAgGGUCg -3' miRNA: 3'- cGCuuUCG---CGGGC----CUCgCGGUgCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 132063 | 0.66 | 0.694303 |
Target: 5'- -aGAGAGCGUCgUGGGGC-CCGCccGCCg -3' miRNA: 3'- cgCUUUCGCGG-GCCUCGcGGUGc-CGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 8671 | 0.66 | 0.745714 |
Target: 5'- aGCG-GAGCaGCCacaucaGGAGCGCCccaAauccgcccgacagaaCGGCCa -3' miRNA: 3'- -CGCuUUCG-CGGg-----CCUCGCGG---U---------------GCCGG- -5' |
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5550 | 3' | -60.8 | NC_001806.1 | + | 140685 | 0.66 | 0.693334 |
Target: 5'- uGCGc-GGCGCCCGc-GUGCCGCGcuggaacGCCc -3' miRNA: 3'- -CGCuuUCGCGGGCcuCGCGGUGC-------CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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