Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5580 | 5' | -58.5 | NC_001806.1 | + | 22884 | 0.7 | 0.611082 |
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Target: 5'- gGGGCUGgcCGGGGCccggcccgccaGCCCCCCGcGGcCGg -3' miRNA: 3'- -UCUGAUa-GUCUCG-----------UGGGGGGC-CCaGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 21951 | 0.66 | 0.795241 |
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Target: 5'- cGGGCccuUCAcggccGGGCAgCCCCggCGGGUCGa -3' miRNA: 3'- -UCUGau-AGU-----CUCGUgGGGG--GCCCAGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 21772 | 0.66 | 0.804028 |
|
Target: 5'- cGGGCgggGUCcgucGAGCGCggcgccggcagCCCCCGGG-CGg -3' miRNA: 3'- -UCUGa--UAGu---CUCGUG-----------GGGGGCCCaGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 17206 | 0.68 | 0.691101 |
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Target: 5'- aGGGCccuuaaauuuUUAGAGCAgCCCCCGcGUCGg -3' miRNA: 3'- -UCUGau--------AGUCUCGUgGGGGGCcCAGC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 17165 | 0.66 | 0.812666 |
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Target: 5'- -cGCUccaAGGGC-CCCCCCGGGaaggCGg -3' miRNA: 3'- ucUGAuagUCUCGuGGGGGGCCCa---GC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 4147 | 0.66 | 0.813521 |
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Target: 5'- cGGCUGUCGcccaggccgccguacAGCACCcgCCCCGGGggCGg -3' miRNA: 3'- uCUGAUAGUc--------------UCGUGG--GGGGCCCa-GC- -5' |
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| 5580 | 5' | -58.5 | NC_001806.1 | + | 3515 | 0.69 | 0.64118 |
|
Target: 5'- cGGGCggcGUCGGGGUcgucGCCCCCCGcGGgggaggCGg -3' miRNA: 3'- -UCUGa--UAGUCUCG----UGGGGGGC-CCa-----GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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