Results 1 - 20 of 93 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5648 | 3' | -57.5 | NC_001806.1 | + | 816 | 0.66 | 0.82334 |
Target: 5'- cCGGggGC-GCCCGcGCaccaccaccgccCCUCGCCCc- -3' miRNA: 3'- -GUCuuCGuUGGGUuCG------------GGAGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 143427 | 0.66 | 0.82334 |
Target: 5'- --cGAGCGGCCC-GGCCCgCGCUCcCa -3' miRNA: 3'- gucUUCGUUGGGuUCGGGaGCGGGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 5503 | 0.66 | 0.805897 |
Target: 5'- gAGGAGCAGCggaggCCGccGGCCCccCGCCCc- -3' miRNA: 3'- gUCUUCGUUG-----GGU--UCGGGa-GCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 27202 | 0.66 | 0.813829 |
Target: 5'- gCAGGAGCcGCgCAuauauacgcuuggAGCCagccCGCCCUCa -3' miRNA: 3'- -GUCUUCGuUGgGU-------------UCGGga--GCGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 75860 | 0.66 | 0.796029 |
Target: 5'- gGGAAcCAACgCCAAGgcggugaCCCUUGCCCUg -3' miRNA: 3'- gUCUUcGUUG-GGUUC-------GGGAGCGGGAg -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 612 | 0.66 | 0.796934 |
Target: 5'- cCAGcccucccCAGCCCcAGCCCUCcccggccccaGCCCUCc -3' miRNA: 3'- -GUCuuc----GUUGGGuUCGGGAG----------CGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 143946 | 0.66 | 0.805897 |
Target: 5'- --cGAGCAcccccGCCCAcGCCCcCGCCCc- -3' miRNA: 3'- gucUUCGU-----UGGGUuCGGGaGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 132687 | 0.66 | 0.805008 |
Target: 5'- uGGggGCcuGGCCCGcggugggGGCCagggUCGUCCUCc -3' miRNA: 3'- gUCuuCG--UUGGGU-------UCGGg---AGCGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 73200 | 0.66 | 0.822484 |
Target: 5'- -cGccGCucGCCCGAGCCCccgggcgcagaccUCGCCCa- -3' miRNA: 3'- guCuuCGu-UGGGUUCGGG-------------AGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 52637 | 0.66 | 0.814703 |
Target: 5'- gCGGgcGCGGCgcgacaaCGAGCCCcCGcCCCUCc -3' miRNA: 3'- -GUCuuCGUUGg------GUUCGGGaGC-GGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 67172 | 0.66 | 0.796934 |
Target: 5'- cCAucAGCuGGCCCcAGgCCUCGCCCa- -3' miRNA: 3'- -GUcuUCG-UUGGGuUCgGGAGCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 4358 | 0.66 | 0.822484 |
Target: 5'- -cGAuGCGGCCCGcggaggccgcgggGGUCCUCGCCg-- -3' miRNA: 3'- guCUuCGUUGGGU-------------UCGGGAGCGGgag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 65444 | 0.66 | 0.812078 |
Target: 5'- aGGGAGCGGgggaugccgcggccCCCGGGUCCUgggggCGCCCg- -3' miRNA: 3'- gUCUUCGUU--------------GGGUUCGGGA-----GCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 147297 | 0.66 | 0.796934 |
Target: 5'- uCAGggGgGuCCCGGGCCCacccuggccgCGCCCc- -3' miRNA: 3'- -GUCuuCgUuGGGUUCGGGa---------GCGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 73442 | 0.66 | 0.796029 |
Target: 5'- cCGGAcGCAcACCCAggccgggguggccGGCCCggccgcCGCCCUg -3' miRNA: 3'- -GUCUuCGU-UGGGU-------------UCGGGa-----GCGGGAg -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 84981 | 0.66 | 0.82334 |
Target: 5'- -cGggGCcuuuucgccGCCCucGCCCagaCGCCCUCc -3' miRNA: 3'- guCuuCGu--------UGGGuuCGGGa--GCGGGAG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 49639 | 0.66 | 0.840083 |
Target: 5'- gGGAcGCGugCCGGGCCCggaGCUC-Cg -3' miRNA: 3'- gUCUuCGUugGGUUCGGGag-CGGGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 66738 | 0.66 | 0.805897 |
Target: 5'- aCAGAAacGCcgcGCCCGGGCCCcccaGCCC-Ca -3' miRNA: 3'- -GUCUU--CGu--UGGGUUCGGGag--CGGGaG- -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 60964 | 0.66 | 0.814703 |
Target: 5'- gCAGuuGCgGACCCAGGCCC-C-CCCa- -3' miRNA: 3'- -GUCuuCG-UUGGGUUCGGGaGcGGGag -5' |
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5648 | 3' | -57.5 | NC_001806.1 | + | 72591 | 0.66 | 0.813829 |
Target: 5'- cCAGucGCcgccaccGACCCGGGCCCgUCGCgacgacagggCCUCc -3' miRNA: 3'- -GUCuuCG-------UUGGGUUCGGG-AGCG----------GGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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