Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5747 | 5' | -53.6 | NC_001806.1 | + | 20089 | 0.7 | 0.84629 |
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Target: 5'- uCCCGUCGUuCGUGGCCggcGCc-GUCUg -3' miRNA: 3'- -GGGCGGCAcGCACCGGaa-UGuuUAGA- -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 1330 | 0.71 | 0.812322 |
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Target: 5'- uCCCGCCGcggggGCGUGGCCa--------- -3' miRNA: 3'- -GGGCGGCa----CGCACCGGaauguuuaga -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 4333 | 0.71 | 0.794298 |
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Target: 5'- aCCGCCGcGCG-GGCCcgGCGGcgCUc -3' miRNA: 3'- gGGCGGCaCGCaCCGGaaUGUUuaGA- -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 103162 | 0.71 | 0.794298 |
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Target: 5'- gCCGCUGUGCGcugGGCCUguaggACGAc--- -3' miRNA: 3'- gGGCGGCACGCa--CCGGAa----UGUUuaga -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 24277 | 0.73 | 0.665864 |
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Target: 5'- gCCCGCCGUGCaGUGcGCCgUGCGc---- -3' miRNA: 3'- -GGGCGGCACG-CAC-CGGaAUGUuuaga -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 135756 | 0.74 | 0.655528 |
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Target: 5'- aCgGCCGUGCGgaaGGCCUgGCGAAgCUg -3' miRNA: 3'- gGgCGGCACGCa--CCGGAaUGUUUaGA- -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 114937 | 0.74 | 0.634807 |
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Target: 5'- aCCGCCGU-CGUGGCCacgGCAAccGUCc -3' miRNA: 3'- gGGCGGCAcGCACCGGaa-UGUU--UAGa -5' |
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| 5747 | 5' | -53.6 | NC_001806.1 | + | 17249 | 1.11 | 0.00401 |
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Target: 5'- cCCCGCCGUGCGUGGCCUUACAAAUCUg -3' miRNA: 3'- -GGGCGGCACGCACCGGAAUGUUUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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