Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5974 | 3' | -57.6 | NC_001806.1 | + | 119031 | 0.66 | 0.828113 |
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Target: 5'- uGGCGGCgcugcgccuUGCGUGGggcUCGGGGgCGaGCUGu -3' miRNA: 3'- -UCGUCG---------GCGCACU---AGUCCC-GCaUGAC- -5' |
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| 5974 | 3' | -57.6 | NC_001806.1 | + | 142193 | 0.73 | 0.449395 |
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Target: 5'- gGGCGGCgGCGUGGUgaGGGGCuucgGCUGc -3' miRNA: 3'- -UCGUCGgCGCACUAg-UCCCGca--UGAC- -5' |
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| 5974 | 3' | -57.6 | NC_001806.1 | + | 144537 | 0.68 | 0.70703 |
|
Target: 5'- cGCGGCCGUGccGAUCGcGGGUGgUGCg- -3' miRNA: 3'- uCGUCGGCGCa-CUAGU-CCCGC-AUGac -5' |
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| 5974 | 3' | -57.6 | NC_001806.1 | + | 147225 | 0.66 | 0.844545 |
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Target: 5'- aGGCGGCCcccGCGggGGUCGGGGCc----- -3' miRNA: 3'- -UCGUCGG---CGCa-CUAGUCCCGcaugac -5' |
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| 5974 | 3' | -57.6 | NC_001806.1 | + | 147768 | 0.68 | 0.69702 |
|
Target: 5'- cGCGGCCGCGgacgcgggGGgccCGGGGCGggggGCg- -3' miRNA: 3'- uCGUCGGCGCa-------CUa--GUCCCGCa---UGac -5' |
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| 5974 | 3' | -57.6 | NC_001806.1 | + | 148166 | 0.66 | 0.819627 |
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Target: 5'- cGGCGGCgccucUGCGUGGgggggcgCGGGGCGUccgGCg- -3' miRNA: 3'- -UCGUCG-----GCGCACUa------GUCCCGCA---UGac -5' |
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| 5974 | 3' | -57.6 | NC_001806.1 | + | 150716 | 0.66 | 0.810972 |
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Target: 5'- aGGCGGcCCGCccuGUgAGGGCGgGCUGg -3' miRNA: 3'- -UCGUC-GGCGcacUAgUCCCGCaUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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