Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5978 | 3' | -50.6 | NC_001806.1 | + | 145518 | 0.7 | 0.940801 |
Target: 5'- uGGcucUCuAUGCUUGGGUcuuacugccuGGGggGGGGGa -3' miRNA: 3'- gCC---AGcUACGAACCCA----------CCCuuUUUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 34772 | 0.7 | 0.940801 |
Target: 5'- cCGG-CGggGCgguucgGGGgggGGGggGGGGGg -3' miRNA: 3'- -GCCaGCuaCGaa----CCCa--CCCuuUUUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 110704 | 0.72 | 0.88037 |
Target: 5'- uGGUCGAcgagGCcgccuucgGGG-GGGAGGAGGGa -3' miRNA: 3'- gCCAGCUa---CGaa------CCCaCCCUUUUUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 69498 | 0.72 | 0.88037 |
Target: 5'- gGGUCGAacgUGUUUGGGUuggcgcGGGAAuacGGGu -3' miRNA: 3'- gCCAGCU---ACGAACCCA------CCCUUuu-UCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 30804 | 0.73 | 0.841225 |
Target: 5'- -cGUCGGUGCccUGGGaGGGAGAcaaGAGGa -3' miRNA: 3'- gcCAGCUACGa-ACCCaCCCUUU---UUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 32023 | 0.77 | 0.656453 |
Target: 5'- gGGUgGG-GCggagGGGUGGGggGGGGGg -3' miRNA: 3'- gCCAgCUaCGaa--CCCACCCuuUUUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 11761 | 0.87 | 0.231171 |
Target: 5'- uGGUCGAUGCUUGGGgagcGGGGAugguggucGAGGGa -3' miRNA: 3'- gCCAGCUACGAACCCa---CCCUU--------UUUCC- -5' |
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5978 | 3' | -50.6 | NC_001806.1 | + | 5295 | 1.12 | 0.006533 |
Target: 5'- cCGGUCGAUGCUUGGGUGGGAAAAAGGa -3' miRNA: 3'- -GCCAGCUACGAACCCACCCUUUUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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