Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 611 | 3' | -53.6 | AC_000017.1 | + | 14276 | 0.69 | 0.434623 |
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Target: 5'- cUCcGCGGUACCUgCgGCCuaccggggggAGAAACAGCa -3' miRNA: 3'- -AGuCGUCGUGGA-GgCGG----------UCUUUGUUG- -5' |
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| 611 | 3' | -53.6 | AC_000017.1 | + | 8727 | 0.7 | 0.408542 |
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Target: 5'- gCGGUugaucuccugaaucuGGCGCCUCUGCguGaAGACGACg -3' miRNA: 3'- aGUCG---------------UCGUGGAGGCGguC-UUUGUUG- -5' |
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| 611 | 3' | -53.6 | AC_000017.1 | + | 17330 | 0.7 | 0.385352 |
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Target: 5'- --cGCGGCAUCUgccaCCGCC-GAGGCGACc -3' miRNA: 3'- aguCGUCGUGGA----GGCGGuCUUUGUUG- -5' |
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| 611 | 3' | -53.6 | AC_000017.1 | + | 5571 | 0.71 | 0.357616 |
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Target: 5'- cUCGuGCGGCGCCUCCuCCAaGGGCAAg -3' miRNA: 3'- -AGU-CGUCGUGGAGGcGGUcUUUGUUg -5' |
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| 611 | 3' | -53.6 | AC_000017.1 | + | 6551 | 0.72 | 0.33131 |
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Target: 5'- -gGGCGGcCGCCUCUGCUGGAccAACGAg -3' miRNA: 3'- agUCGUC-GUGGAGGCGGUCU--UUGUUg -5' |
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| 611 | 3' | -53.6 | AC_000017.1 | + | 18854 | 0.72 | 0.290694 |
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Target: 5'- cUCAGCAGCuCCUCUGgCGGcGACAu- -3' miRNA: 3'- -AGUCGUCGuGGAGGCgGUCuUUGUug -5' |
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| 611 | 3' | -53.6 | AC_000017.1 | + | 9837 | 1.1 | 0.000627 |
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Target: 5'- aUCAGCAGCACCUCCGCCAGAAACAACc -3' miRNA: 3'- -AGUCGUCGUGGAGGCGGUCUUUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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