Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6344 | 3' | -54.8 | NC_001847.1 | + | 120653 | 0.68 | 0.805627 |
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Target: 5'- -uUGGCCGCGUuuguacACA-GCUUcGGUGAg- -3' miRNA: 3'- cuACCGGCGCG------UGUaCGAA-CCACUag -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 126782 | 0.73 | 0.509264 |
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Target: 5'- ---uGCCGCGCccuACGUGUUUGGUcGAUCg -3' miRNA: 3'- cuacCGGCGCG---UGUACGAACCA-CUAG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 127246 | 0.67 | 0.865061 |
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Target: 5'- --cGGCCGcCGCGCGccccgcgcgGCUaGGUGGUUu -3' miRNA: 3'- cuaCCGGC-GCGUGUa--------CGAaCCACUAG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 128081 | 0.67 | 0.865061 |
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Target: 5'- --cGGCuCGC-CGCGUGCcUUGGUGGc- -3' miRNA: 3'- cuaCCG-GCGcGUGUACG-AACCACUag -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 128832 | 0.66 | 0.880139 |
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Target: 5'- cGUGGCCGCGCGCAU-CgaGGccGAccUCg -3' miRNA: 3'- cUACCGGCGCGUGUAcGaaCCa-CU--AG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 131429 | 0.66 | 0.892905 |
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Target: 5'- cGUGGCCgGCGCGCGcgauUGCcgcgccuaccugUGGUGcgCg -3' miRNA: 3'- cUACCGG-CGCGUGU----ACGa-----------ACCACuaG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 133423 | 0.69 | 0.76779 |
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Target: 5'- -cUGGCCGCgGCGCG-GCggcGGcUGAUCg -3' miRNA: 3'- cuACCGGCG-CGUGUaCGaa-CC-ACUAG- -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 134519 | 0.73 | 0.5295 |
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Target: 5'- --cGGCCGCgGCGCAUGU--GGUGGUa -3' miRNA: 3'- cuaCCGGCG-CGUGUACGaaCCACUAg -5' |
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| 6344 | 3' | -54.8 | NC_001847.1 | + | 135012 | 1.09 | 0.002473 |
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Target: 5'- aGAUGGCCGCGCACAUGCUUGGUGAUCu -3' miRNA: 3'- -CUACCGGCGCGUGUACGAACCACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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