Results 1 - 20 of 127 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6673 | 3' | -58.8 | NC_001847.1 | + | 135020 | 0.66 | 0.771292 |
Target: 5'- aGGCC-CgcggGCGg-GGCCGGGGCg--- -3' miRNA: 3'- gCCGGaGa---UGCgaCCGGCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 11195 | 0.66 | 0.771292 |
Target: 5'- uGGUCcgCgcgGgGCUGGUCGAGGUUGa- -3' miRNA: 3'- gCCGGa-Ga--UgCGACCGGCUCCGAUac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 100893 | 0.66 | 0.742771 |
Target: 5'- gCGGCCggCgggagACGg-GGUCGGGGCUcgGg -3' miRNA: 3'- -GCCGGa-Ga----UGCgaCCGGCUCCGAuaC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 27910 | 0.66 | 0.752386 |
Target: 5'- cCGGCCgcgcUUGCGgaGGCCaGGGCg--- -3' miRNA: 3'- -GCCGGa---GAUGCgaCCGGcUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 97698 | 0.66 | 0.761896 |
Target: 5'- aGGCCcgcugCUGCGCUcgccgucgucGGCgGGGGCa--- -3' miRNA: 3'- gCCGGa----GAUGCGA----------CCGgCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 55508 | 0.66 | 0.733061 |
Target: 5'- gGGCC-CUGCuGCUGGCggcgcccgccgCGGGGCg--- -3' miRNA: 3'- gCCGGaGAUG-CGACCG-----------GCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 121353 | 0.66 | 0.761896 |
Target: 5'- uGGUUUCUGCGCggcGGgCGGGGUg--- -3' miRNA: 3'- gCCGGAGAUGCGa--CCgGCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 16681 | 0.66 | 0.752386 |
Target: 5'- gGGCCUUgcgcCGCaGGCaCGAGGCc--- -3' miRNA: 3'- gCCGGAGau--GCGaCCG-GCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 34756 | 0.66 | 0.769423 |
Target: 5'- gCGGCCgagcuggcgcggCUGCGCgccgcGGCCGcGGCg--- -3' miRNA: 3'- -GCCGGa-----------GAUGCGa----CCGGCuCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 945 | 0.66 | 0.742771 |
Target: 5'- uCGGCCcgcgcgcgccgcUCcACGCUGcGCCGGGcGuCUAUGc -3' miRNA: 3'- -GCCGG------------AGaUGCGAC-CGGCUC-C-GAUAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 88828 | 0.66 | 0.733061 |
Target: 5'- gCGGCCgugCUGCGCUcGGCgCuacGGCUGg- -3' miRNA: 3'- -GCCGGa--GAUGCGA-CCG-Gcu-CCGAUac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 133403 | 0.66 | 0.733061 |
Target: 5'- uGGaguaCCUCUGCGCgcggcUGGCCGcGGCg--- -3' miRNA: 3'- gCC----GGAGAUGCG-----ACCGGCuCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 34809 | 0.66 | 0.742771 |
Target: 5'- gCGGCCgcugGCGCggcaGGCCGAccgGGCgcUGg -3' miRNA: 3'- -GCCGGaga-UGCGa---CCGGCU---CCGauAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 93108 | 0.66 | 0.733061 |
Target: 5'- aCGaGCCagUCUACguaccgggcgaaGCUGGCCG-GGCUGc- -3' miRNA: 3'- -GC-CGG--AGAUG------------CGACCGGCuCCGAUac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 124592 | 0.66 | 0.771292 |
Target: 5'- gGGCC-CgGCGCcGGCCGGGcGCg--- -3' miRNA: 3'- gCCGGaGaUGCGaCCGGCUC-CGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 129322 | 0.66 | 0.742771 |
Target: 5'- gGGCCUgCcggGCGCggGGCCgGGGGCg--- -3' miRNA: 3'- gCCGGA-Ga--UGCGa-CCGG-CUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 83040 | 0.66 | 0.733061 |
Target: 5'- gCGGCCgCUaguGCGCUugGGCCGcGGCg--- -3' miRNA: 3'- -GCCGGaGA---UGCGA--CCGGCuCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 39718 | 0.66 | 0.736956 |
Target: 5'- uGG-CUCUGCGCUGucgggccuuggcuccGCCGAGGaggAUGc -3' miRNA: 3'- gCCgGAGAUGCGAC---------------CGGCUCCga-UAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 111286 | 0.66 | 0.752386 |
Target: 5'- gGGCCgggggCgGCGCUcGGCCgGGGGCg--- -3' miRNA: 3'- gCCGGa----GaUGCGA-CCGG-CUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 105034 | 0.66 | 0.742771 |
Target: 5'- aGGCCgccagCgccgcgGCGCUGGgCGcGGGCgUGUGg -3' miRNA: 3'- gCCGGa----Ga-----UGCGACCgGC-UCCG-AUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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