Results 1 - 20 of 127 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6673 | 3' | -58.8 | NC_001847.1 | + | 116274 | 1.07 | 0.001772 |
Target: 5'- uCGGCCUCUACGCUGGCCGAGGCUAUGc -3' miRNA: 3'- -GCCGGAGAUGCGACCGGCUCCGAUAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 43826 | 0.76 | 0.250479 |
Target: 5'- gCGGCCgcuUCUuugccGCGCUGGCgcugcuggagccCGAGGCUGUGu -3' miRNA: 3'- -GCCGG---AGA-----UGCGACCG------------GCUCCGAUAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 14145 | 0.75 | 0.262675 |
Target: 5'- cCGGCCggCUGCGCagcggcGGCCGGGGCg--- -3' miRNA: 3'- -GCCGGa-GAUGCGa-----CCGGCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 74125 | 0.75 | 0.288508 |
Target: 5'- cCGGCCguggugCUGCGCgugGcGCCGGGGCgaaugAUGg -3' miRNA: 3'- -GCCGGa-----GAUGCGa--C-CGGCUCCGa----UAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 86029 | 0.74 | 0.316296 |
Target: 5'- aCGGCaCUgCUGCGCUGGUugggCGAGGCgcUGg -3' miRNA: 3'- -GCCG-GA-GAUGCGACCG----GCUCCGauAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 81560 | 0.73 | 0.346055 |
Target: 5'- cCGGCCccgcgcagCUGCGCggGGCCGGcGGCUAc- -3' miRNA: 3'- -GCCGGa-------GAUGCGa-CCGGCU-CCGAUac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 60038 | 0.73 | 0.361669 |
Target: 5'- uCGGCCUCUuCGUcgGGCgCGAGGCg--- -3' miRNA: 3'- -GCCGGAGAuGCGa-CCG-GCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 11594 | 0.73 | 0.377768 |
Target: 5'- -uGCUUCUGCGCUGGCCGGcGCguccucgAUGg -3' miRNA: 3'- gcCGGAGAUGCGACCGGCUcCGa------UAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 118227 | 0.72 | 0.385995 |
Target: 5'- cCGGCgUCUGCGCcgagcGGCCcGGGCUGg- -3' miRNA: 3'- -GCCGgAGAUGCGa----CCGGcUCCGAUac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 29360 | 0.72 | 0.420065 |
Target: 5'- gCGGCCcCggcgGCGCUGcgcGCCGAGGCg--- -3' miRNA: 3'- -GCCGGaGa---UGCGAC---CGGCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 132173 | 0.72 | 0.420065 |
Target: 5'- gCGGCCcCggcgGCGCUGcgcGCCGAGGCg--- -3' miRNA: 3'- -GCCGGaGa---UGCGAC---CGGCUCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 116122 | 0.71 | 0.436873 |
Target: 5'- uCGGCCUUUcggccggGCGCUGGggcgcgcggcCCGAGGCgcUGg -3' miRNA: 3'- -GCCGGAGA-------UGCGACC----------GGCUCCGauAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 17327 | 0.71 | 0.44136 |
Target: 5'- aCGGCCgcguagccggcgccuUCUAgCGCUGGCUGGGGUUc-- -3' miRNA: 3'- -GCCGG---------------AGAU-GCGACCGGCUCCGAuac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 76388 | 0.71 | 0.455892 |
Target: 5'- aCGGCCUCgggggggGCGCcgggGGCCGcGGCg--- -3' miRNA: 3'- -GCCGGAGa------UGCGa---CCGGCuCCGauac -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 132290 | 0.71 | 0.455892 |
Target: 5'- uCGGCCUggGCGCUGGCgCGcGcGCUGUu -3' miRNA: 3'- -GCCGGAgaUGCGACCG-GCuC-CGAUAc -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 29477 | 0.71 | 0.455892 |
Target: 5'- uCGGCCUggGCGCUGGCgCGcGcGCUGUu -3' miRNA: 3'- -GCCGGAgaUGCGACCG-GCuC-CGAUAc -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 130529 | 0.71 | 0.465104 |
Target: 5'- gGGCCgcccgCgccgaGCUGGCCGA-GCUGUGg -3' miRNA: 3'- gCCGGa----Gaug--CGACCGGCUcCGAUAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 27716 | 0.71 | 0.465104 |
Target: 5'- gGGCCgcccgCgccgaGCUGGCCGA-GCUGUGg -3' miRNA: 3'- gCCGGa----Gaug--CGACCGGCUcCGAUAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 50373 | 0.71 | 0.474411 |
Target: 5'- cCGGCgUCgcccUGCUGGCCGuGGCcAUGc -3' miRNA: 3'- -GCCGgAGau--GCGACCGGCuCCGaUAC- -5' |
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6673 | 3' | -58.8 | NC_001847.1 | + | 36938 | 0.71 | 0.483812 |
Target: 5'- uGuGCUUCU-CGCUGGCCGAgcgggGGCUGc- -3' miRNA: 3'- gC-CGGAGAuGCGACCGGCU-----CCGAUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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