Results 1 - 20 of 78 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6678 | 3' | -56 | NC_001847.1 | + | 11541 | 0.66 | 0.902344 |
Target: 5'- uGCcGCCuuGGCUGGGcuugcaaagCCCg -3' miRNA: 3'- -CGuCGGggCCGACCCauauaua--GGGg -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 75286 | 0.66 | 0.901708 |
Target: 5'- cGCGGCCCuCGGCgaccgcgccUGGccg-GUG-CCCCg -3' miRNA: 3'- -CGUCGGG-GCCG---------ACCcauaUAUaGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 22856 | 0.66 | 0.901708 |
Target: 5'- cGCGGUCCUGGguuucagcuCUGGGgg-GUcgCCgCCg -3' miRNA: 3'- -CGUCGGGGCC---------GACCCauaUAuaGG-GG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 125669 | 0.66 | 0.901708 |
Target: 5'- cGCGGUCCUGGguuucagcuCUGGGgg-GUcgCCgCCg -3' miRNA: 3'- -CGUCGGGGCC---------GACCCauaUAuaGG-GG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 6985 | 0.66 | 0.901068 |
Target: 5'- aUAGCUugCCGGCgUGGGUAguuUGUAUaugcacacccgcgCCCCu -3' miRNA: 3'- cGUCGG--GGCCG-ACCCAU---AUAUA-------------GGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 106897 | 0.66 | 0.89521 |
Target: 5'- uCGGCCUCGGCcucagcgucGGcGUcgGUGcUCCCCu -3' miRNA: 3'- cGUCGGGGCCGa--------CC-CAuaUAU-AGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 4084 | 0.66 | 0.89521 |
Target: 5'- uCGGCCUCGGCcucagcgucGGcGUcgGUGcUCCCCu -3' miRNA: 3'- cGUCGGGGCCGa--------CC-CAuaUAU-AGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 13955 | 0.66 | 0.893882 |
Target: 5'- uGCGGCUgCGGCccgUGGGagcccg-CCCCu -3' miRNA: 3'- -CGUCGGgGCCG---ACCCauauauaGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 75646 | 0.66 | 0.891199 |
Target: 5'- uGCGGCCucggcgcacccccugCCGGCUGGGgacuacGUGUAcgUgCCg -3' miRNA: 3'- -CGUCGG---------------GGCCGACCCa-----UAUAUa-GgGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 11586 | 0.66 | 0.889162 |
Target: 5'- gGCGGCCCCGGCccaag------CCCCg -3' miRNA: 3'- -CGUCGGGGCCGacccauauauaGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 64449 | 0.66 | 0.888479 |
Target: 5'- gGCGGCCaCGGCcagccccGGGcccgcgGUCCCCu -3' miRNA: 3'- -CGUCGGgGCCGa------CCCauaua-UAGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 93439 | 0.66 | 0.888479 |
Target: 5'- --cGCCCUGGCccucGGUGUAgcccagcUCCCCc -3' miRNA: 3'- cguCGGGGCCGac--CCAUAUau-----AGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 123622 | 0.66 | 0.888479 |
Target: 5'- cGCAGCaCCCGGC-GGcUAcGUgccaGUUCCCg -3' miRNA: 3'- -CGUCG-GGGCCGaCCcAUaUA----UAGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 16245 | 0.66 | 0.887105 |
Target: 5'- uCGGCCCCGGCcccGGccccgg-CCCCg -3' miRNA: 3'- cGUCGGGGCCGac-CCauauauaGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 36456 | 0.66 | 0.885028 |
Target: 5'- uCGGCCCCGGCUucGGccccggcuucggCCCCg -3' miRNA: 3'- cGUCGGGGCCGA--CCcauauaua----GGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 8737 | 0.66 | 0.877236 |
Target: 5'- cCAGCCCCGGCaaaggcaaGGUAagcgcauccgCCCCg -3' miRNA: 3'- cGUCGGGGCCGac------CCAUauaua-----GGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 111550 | 0.66 | 0.877236 |
Target: 5'- cCAGCCCCGGCaaaggcaaGGUAagcgcauccgCCCCg -3' miRNA: 3'- cGUCGGGGCCGac------CCAUauaua-----GGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 59282 | 0.66 | 0.875065 |
Target: 5'- -gAGCCCCGGCggcGGGaaaccguaCCCa -3' miRNA: 3'- cgUCGGGGCCGa--CCCauauauagGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 28307 | 0.66 | 0.871401 |
Target: 5'- aGCGGCCCCgcgagaggcgGGCgccGGGcccggcgCCCCg -3' miRNA: 3'- -CGUCGGGG----------CCGa--CCCauauauaGGGG- -5' |
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6678 | 3' | -56 | NC_001847.1 | + | 131120 | 0.66 | 0.871401 |
Target: 5'- aGCGGCCCCgcgagaggcgGGCgccGGGcccggcgCCCCg -3' miRNA: 3'- -CGUCGGGG----------CCGa--CCCauauauaGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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