Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6730 | 3' | -54 | NC_001875.2 | + | 118727 | 0.66 | 0.960851 |
Target: 5'- cGGUUaccugcgaaGCAUCCaGCUG-CGGcacauGGUGGCGg -3' miRNA: 3'- -CCAG---------UGUAGG-CGGCuGCCu----UUACCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 118227 | 0.66 | 0.960851 |
Target: 5'- uGGUgGCAgcagucUCCGuuGuCGGAcaaucgugcgGGUGGCGc -3' miRNA: 3'- -CCAgUGU------AGGCggCuGCCU----------UUACCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 113531 | 0.66 | 0.960851 |
Target: 5'- -aUCGCGcgCCGCUGAcCGGGcAAUGaGCGa -3' miRNA: 3'- ccAGUGUa-GGCGGCU-GCCU-UUAC-CGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 30197 | 0.66 | 0.957175 |
Target: 5'- uGG-CGcCGUUCGCCgcGACGGccguuGUGGCGa -3' miRNA: 3'- -CCaGU-GUAGGCGG--CUGCCuu---UACCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 126097 | 0.66 | 0.957175 |
Target: 5'- uGUCGCuggCCGCCGA-----AUGGCGg -3' miRNA: 3'- cCAGUGua-GGCGGCUgccuuUACCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 95355 | 0.66 | 0.95327 |
Target: 5'- cGGcgCACGUgcaCgCGUCGGCGGAAAacGGCGu -3' miRNA: 3'- -CCa-GUGUA---G-GCGGCUGCCUUUa-CCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 11137 | 0.66 | 0.95327 |
Target: 5'- -uUCGCGaagugaUUGCCGACGGGGA-GGCGu -3' miRNA: 3'- ccAGUGUa-----GGCGGCUGCCUUUaCCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 93243 | 0.66 | 0.949131 |
Target: 5'- uGUCGCGUCCGCCGcucaucagcaACGuGuuuuuguuuGcgGGCGg -3' miRNA: 3'- cCAGUGUAGGCGGC----------UGC-Cu--------UuaCCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 4494 | 0.66 | 0.949131 |
Target: 5'- aGG-CACAcguugcgucugcUCCGCUG-CGGAAAgcGGCGc -3' miRNA: 3'- -CCaGUGU------------AGGCGGCuGCCUUUa-CCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 110051 | 0.66 | 0.944757 |
Target: 5'- cGUUaaACGUUCGCgGcGCGGGGAUgGGCGg -3' miRNA: 3'- cCAG--UGUAGGCGgC-UGCCUUUA-CCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 104304 | 0.66 | 0.944757 |
Target: 5'- cGGaCGCGUCuauCGCC-ACGGGAAUGGg- -3' miRNA: 3'- -CCaGUGUAG---GCGGcUGCCUUUACCgc -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 77654 | 0.66 | 0.944757 |
Target: 5'- cGGUCACGUCguuggacgCGcCCGGCGcGuug-GGCGa -3' miRNA: 3'- -CCAGUGUAG--------GC-GGCUGC-CuuuaCCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 14507 | 0.67 | 0.940143 |
Target: 5'- --gCGCuuauUCCGCUGuCGGggGaacuUGGCGg -3' miRNA: 3'- ccaGUGu---AGGCGGCuGCCuuU----ACCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 111762 | 0.67 | 0.930189 |
Target: 5'- -aUCACGUgCGCCGACGac--UGGUGu -3' miRNA: 3'- ccAGUGUAgGCGGCUGCcuuuACCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 41953 | 0.68 | 0.907363 |
Target: 5'- cGG-CGCGcgUGCCGGCGGAAAUcacaagcaugGGCGu -3' miRNA: 3'- -CCaGUGUagGCGGCUGCCUUUA----------CCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 74317 | 0.68 | 0.907363 |
Target: 5'- --aCGCGUCCaaGCUGGCGGcGGcgGGCGc -3' miRNA: 3'- ccaGUGUAGG--CGGCUGCC-UUuaCCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 29099 | 0.68 | 0.906743 |
Target: 5'- --gCACAUugagcggCCgGCCGuugaaGCGGAGGUGGCGg -3' miRNA: 3'- ccaGUGUA-------GG-CGGC-----UGCCUUUACCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 43980 | 0.68 | 0.900409 |
Target: 5'- uGGUCGCcgaCCGCC-ACGGAcagcccgAcgGGCGc -3' miRNA: 3'- -CCAGUGua-GGCGGcUGCCU-------UuaCCGC- -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 111798 | 0.68 | 0.894506 |
Target: 5'- cGGUgCACG-CgGCCGACGGcgccUGGCu -3' miRNA: 3'- -CCA-GUGUaGgCGGCUGCCuuu-ACCGc -5' |
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6730 | 3' | -54 | NC_001875.2 | + | 65432 | 0.68 | 0.894506 |
Target: 5'- cGUCcgGCGUgcaCCGCUGGCGGucGGUGGCc -3' miRNA: 3'- cCAG--UGUA---GGCGGCUGCCu-UUACCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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