Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7108 | 5' | -61 | NC_001895.1 | + | 10397 | 0.7 | 0.138968 |
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Target: 5'- cGCGUgcagACCGGCGGCAUgugCACCgACUGg- -3' miRNA: 3'- uCGCG----UGGCCGCCGUA---GUGG-UGGCag -5' |
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| 7108 | 5' | -61 | NC_001895.1 | + | 6679 | 0.71 | 0.135151 |
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Target: 5'- cGGCgGUGCCGuGUGGCAgCACCGCCcccaGUCa -3' miRNA: 3'- -UCG-CGUGGC-CGCCGUaGUGGUGG----CAG- -5' |
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| 7108 | 5' | -61 | NC_001895.1 | + | 17823 | 0.71 | 0.123925 |
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Target: 5'- aGGCaCGCUGGCGGCAUCguugcagGCCAUCGc- -3' miRNA: 3'- -UCGcGUGGCCGCCGUAG-------UGGUGGCag -5' |
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| 7108 | 5' | -61 | NC_001895.1 | + | 12783 | 0.72 | 0.101689 |
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Target: 5'- -cCGCGCCGGgaaggcggaagguCGGCAUUACCacgaugGCCGUCg -3' miRNA: 3'- ucGCGUGGCC-------------GCCGUAGUGG------UGGCAG- -5' |
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| 7108 | 5' | -61 | NC_001895.1 | + | 5487 | 0.73 | 0.083491 |
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Target: 5'- cAGCGCACCGGCaauaucgccGGUAUCcagacGCCacagcaugACCGUCa -3' miRNA: 3'- -UCGCGUGGCCG---------CCGUAG-----UGG--------UGGCAG- -5' |
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| 7108 | 5' | -61 | NC_001895.1 | + | 23052 | 0.74 | 0.078826 |
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Target: 5'- gGGCGCACCGGaUGuaGUCACCAuCCG-Cg -3' miRNA: 3'- -UCGCGUGGCC-GCcgUAGUGGU-GGCaG- -5' |
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| 7108 | 5' | -61 | NC_001895.1 | + | 20640 | 0.78 | 0.035861 |
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Target: 5'- uGCGCACCGGCuGCAUCAUCuCCGa- -3' miRNA: 3'- uCGCGUGGCCGcCGUAGUGGuGGCag -5' |
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| 7108 | 5' | -61 | NC_001895.1 | + | 5744 | 1.09 | 0.000126 |
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Target: 5'- cAGCGCACCGGCGGCAUCACCACCGUCa -3' miRNA: 3'- -UCGCGUGGCCGCCGUAGUGGUGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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