Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7114 | 3' | -59 | NC_001895.1 | + | 10489 | 0.66 | 0.408268 |
Target: 5'- uCCugCgcgaugggucaGCCACUgaaGCCAGucgguGCacauGCCGCCg -3' miRNA: 3'- -GGugG-----------UGGUGA---UGGUCu----CGg---CGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 1392 | 0.66 | 0.408268 |
Target: 5'- gCGCCACCuAUgagGCCAGccgcagcgaggaAGCCaGCCacGCCg -3' miRNA: 3'- gGUGGUGG-UGa--UGGUC------------UCGG-CGG--CGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 1306 | 0.66 | 0.389892 |
Target: 5'- cUCACCGCCGgUAUCAGcaCC-CCGCUg -3' miRNA: 3'- -GGUGGUGGUgAUGGUCucGGcGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 25013 | 0.66 | 0.389892 |
Target: 5'- cUCACCAaagaauaacuCCGCUucaAUCAGGGCUguaaacauGCCGCUa -3' miRNA: 3'- -GGUGGU----------GGUGA---UGGUCUCGG--------CGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 21665 | 0.66 | 0.372065 |
Target: 5'- aCCACgGCCACaACCGGccAGCUaauaGCC-CCg -3' miRNA: 3'- -GGUGgUGGUGaUGGUC--UCGG----CGGcGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 13690 | 0.67 | 0.338111 |
Target: 5'- --uUCGggUCAUUGcCCAGcGCCGCCGCCa -3' miRNA: 3'- gguGGU--GGUGAU-GGUCuCGGCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 6495 | 0.67 | 0.329982 |
Target: 5'- gCGCCACCGC--CCAGcGCauaucCCGCCa -3' miRNA: 3'- gGUGGUGGUGauGGUCuCGgc---GGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 2910 | 0.67 | 0.321999 |
Target: 5'- gCACggaaaACCcCUGCCAGuacAGCagCGCCGCCu -3' miRNA: 3'- gGUGg----UGGuGAUGGUC---UCG--GCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 5977 | 0.68 | 0.306467 |
Target: 5'- uUCACCGUCACUgACCGGcguGCCGCC-CUg -3' miRNA: 3'- -GGUGGUGGUGA-UGGUCu--CGGCGGcGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 11883 | 0.68 | 0.306467 |
Target: 5'- -gGCCgACCGCgugACCGuGcAGuCUGCCGCCa -3' miRNA: 3'- ggUGG-UGGUGa--UGGU-C-UC-GGCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 13581 | 0.68 | 0.298918 |
Target: 5'- -uGCCGCC-CUugUAGAcUCGUCGCCu -3' miRNA: 3'- ggUGGUGGuGAugGUCUcGGCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 7680 | 0.68 | 0.291515 |
Target: 5'- aCCACCggcaACCACa--CAGAuaaCCGUCGCCa -3' miRNA: 3'- -GGUGG----UGGUGaugGUCUc--GGCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 21155 | 0.68 | 0.284256 |
Target: 5'- gCCAgCucaGCCGCUGCCGugcuGAuGaCUGCCGCCu -3' miRNA: 3'- -GGUgG---UGGUGAUGGU----CU-C-GGCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 4655 | 0.69 | 0.26879 |
Target: 5'- aCGCCGCCGgUGCUucauugcGGuauuucugcagccAGCCGaCCGCCa -3' miRNA: 3'- gGUGGUGGUgAUGG-------UC-------------UCGGC-GGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 12832 | 0.69 | 0.254667 |
Target: 5'- uCCGCCuucccggcgcgguuGCCGCUGCCAGcuUUGCUGCa -3' miRNA: 3'- -GGUGG--------------UGGUGAUGGUCucGGCGGCGg -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 5786 | 0.69 | 0.243685 |
Target: 5'- -aGCUgaaACCGaaGCCGGAGCCGCaGCCc -3' miRNA: 3'- ggUGG---UGGUgaUGGUCUCGGCGgCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 21994 | 0.69 | 0.243685 |
Target: 5'- -gACaCACUug-ACCAGA-CCGCCGCCa -3' miRNA: 3'- ggUG-GUGGugaUGGUCUcGGCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 28919 | 0.71 | 0.181871 |
Target: 5'- ---aCACCAUUGCCGguauuGAGCguuCGCCGCCg -3' miRNA: 3'- ggugGUGGUGAUGGU-----CUCG---GCGGCGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 12744 | 0.71 | 0.176999 |
Target: 5'- uUAUCACCACUGCCGGuGCaGCaaaGCUg -3' miRNA: 3'- gGUGGUGGUGAUGGUCuCGgCGg--CGG- -5' |
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7114 | 3' | -59 | NC_001895.1 | + | 20718 | 0.72 | 0.154344 |
Target: 5'- gCCAgCGCCAUguucagcgugACCGGcGuuGCCGCCu -3' miRNA: 3'- -GGUgGUGGUGa---------UGGUCuCggCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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