Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7166 | 3' | -61.3 | NC_001900.1 | + | 33093 | 0.66 | 0.36732 |
Target: 5'- uCCUcGUCCAGCGCGGCgau--CAGCUu -3' miRNA: 3'- cGGA-CGGGUCGCGUCGaggcuGUCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 26443 | 0.66 | 0.36732 |
Target: 5'- aUCUGCUCGGUGUAGUcCUGGCcgaAGCUg -3' miRNA: 3'- cGGACGGGUCGCGUCGaGGCUG---UCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 21025 | 0.66 | 0.36732 |
Target: 5'- gGCCUGUgaCGGUgGCGGCUCCGcaccCGGUUa -3' miRNA: 3'- -CGGACGg-GUCG-CGUCGAGGCu---GUCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 17088 | 0.66 | 0.358879 |
Target: 5'- gGCCUcacCCCGGUcuuccaGCAGCUCgGugGGUUg -3' miRNA: 3'- -CGGAc--GGGUCG------CGUCGAGgCugUCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 31306 | 0.66 | 0.355541 |
Target: 5'- cGCCUGCagcgcagugguaAGCGCAGUgCCGAgGGUc -3' miRNA: 3'- -CGGACGgg----------UCGCGUCGaGGCUgUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 23315 | 0.66 | 0.342409 |
Target: 5'- cGCgUGCgCCggGGCGCGGC-CCGcguCGGCg -3' miRNA: 3'- -CGgACG-GG--UCGCGUCGaGGCu--GUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 23134 | 0.66 | 0.342409 |
Target: 5'- cCCUGaCCgGGCGCAGC-CCG--GGCg -3' miRNA: 3'- cGGAC-GGgUCGCGUCGaGGCugUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 32100 | 0.66 | 0.334381 |
Target: 5'- aCCgGCuCCAGCGCcgaacgcugacAGCagCGACGGCa -3' miRNA: 3'- cGGaCG-GGUCGCG-----------UCGagGCUGUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 46954 | 0.66 | 0.334381 |
Target: 5'- cGCgCUGaaguaCAGCGaCAGgUUCGACAGCUu -3' miRNA: 3'- -CG-GACgg---GUCGC-GUCgAGGCUGUCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 40660 | 0.66 | 0.332 |
Target: 5'- uGCCgUGCCacucccaguccgcaCGGUGCAGCUCCGG-GGUg -3' miRNA: 3'- -CGG-ACGG--------------GUCGCGUCGAGGCUgUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 30474 | 0.66 | 0.326491 |
Target: 5'- uCCUGCuCCAGCGCAGCcgaGuACuGCUu -3' miRNA: 3'- cGGACG-GGUCGCGUCGaggC-UGuCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 34670 | 0.67 | 0.311129 |
Target: 5'- aGUCcGCCCAGUGCAucaucgccauGCgagCCGACcGGCg -3' miRNA: 3'- -CGGaCGGGUCGCGU----------CGa--GGCUG-UCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 6314 | 0.67 | 0.289127 |
Target: 5'- aCCUGgUCGGCGCAGCcgcaCCGGCcacGGCc -3' miRNA: 3'- cGGACgGGUCGCGUCGa---GGCUG---UCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 33272 | 0.67 | 0.288415 |
Target: 5'- gGCCUucugGCCCAGCuucagcaGCGGCUucagcUCGACGGUc -3' miRNA: 3'- -CGGA----CGGGUCG-------CGUCGA-----GGCUGUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 22135 | 0.67 | 0.288415 |
Target: 5'- aGCUcGCUCAGCGCggugaaacugaGGCUgaaggccagaccgCCGACGGCg -3' miRNA: 3'- -CGGaCGGGUCGCG-----------UCGA-------------GGCUGUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 47859 | 0.67 | 0.28207 |
Target: 5'- uGCCUGCguugCCGGUGCcuaaaccgccccGGCcgcuUCCGGCGGCc -3' miRNA: 3'- -CGGACG----GGUCGCG------------UCG----AGGCUGUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 48710 | 0.68 | 0.275149 |
Target: 5'- aGCCgcGCUUcaGGCGCGGCgcuagaUCCGGCGGUUg -3' miRNA: 3'- -CGGa-CGGG--UCGCGUCG------AGGCUGUCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 25898 | 0.68 | 0.261717 |
Target: 5'- aUCUGCUCGGC-CGGCUCaaCGACGGUc -3' miRNA: 3'- cGGACGGGUCGcGUCGAG--GCUGUCGa -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 32408 | 0.68 | 0.255204 |
Target: 5'- gGCCUugUCGGCGUcggAGC-CCGACAGCUc -3' miRNA: 3'- -CGGAcgGGUCGCG---UCGaGGCUGUCGA- -5' |
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7166 | 3' | -61.3 | NC_001900.1 | + | 42744 | 0.68 | 0.248824 |
Target: 5'- gGCCgUGgCCGGUGCGGCUgcgCCGACcaGGUUg -3' miRNA: 3'- -CGG-ACgGGUCGCGUCGA---GGCUG--UCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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