Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7166 | 3' | -61.3 | NC_001900.1 | + | 882 | 0.69 | 0.213277 |
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Target: 5'- gGCCgcaGCCCGacccaacauucGCGaauGGCUUCGACAGCa -3' miRNA: 3'- -CGGa--CGGGU-----------CGCg--UCGAGGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 2567 | 0.69 | 0.207795 |
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Target: 5'- aGCC-GCUgagGGCGUAGC-CCGACGGCg -3' miRNA: 3'- -CGGaCGGg--UCGCGUCGaGGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 25219 | 0.7 | 0.182182 |
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Target: 5'- gGCCUGgccuucccagcCCCAGCGCcggaagccAGCgCCGAUAGCc -3' miRNA: 3'- -CGGAC-----------GGGUCGCG--------UCGaGGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 22660 | 0.72 | 0.124799 |
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Target: 5'- cGCUgcgGaCCCAGCGCAGCggUCGAguGCa -3' miRNA: 3'- -CGGa--C-GGGUCGCGUCGa-GGCUguCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 25472 | 0.76 | 0.073448 |
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Target: 5'- gGCCUGagccgCCAGCGUGGCUCCcACGGCc -3' miRNA: 3'- -CGGACg----GGUCGCGUCGAGGcUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 43458 | 1.08 | 0.000221 |
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Target: 5'- uGCCUGCCCAGCGCAGCUCCGACAGCUc -3' miRNA: 3'- -CGGACGGGUCGCGUCGAGGCUGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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