Results 1 - 20 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7167 | 3' | -58.2 | NC_001900.1 | + | 41725 | 0.66 | 0.568813 |
Target: 5'- gGUucuCUGA-GGCCACGUCGGcgucGGAGGUa -3' miRNA: 3'- -CGu--GGCUgCCGGUGUAGCC----CUUCCGg -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 20586 | 0.66 | 0.567751 |
Target: 5'- --uCCGACGG--ACAUCuGGGAguggaugGGGCCg -3' miRNA: 3'- cguGGCUGCCggUGUAG-CCCU-------UCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 8777 | 0.66 | 0.565628 |
Target: 5'- cGCGCCGACgugaaggaguucgaGGCaUACGUCGacuccuGGGGCCg -3' miRNA: 3'- -CGUGGCUG--------------CCG-GUGUAGCcc----UUCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 23585 | 0.66 | 0.558213 |
Target: 5'- cGgGCCGugGgaGCCACGcUGGcGGcucAGGCCg -3' miRNA: 3'- -CgUGGCugC--CGGUGUaGCC-CU---UCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 42412 | 0.66 | 0.558213 |
Target: 5'- cGUAUCGGCaggcaGGCCAgGcccgaggggaUCGGGcuGGCCc -3' miRNA: 3'- -CGUGGCUG-----CCGGUgU----------AGCCCuuCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 12620 | 0.66 | 0.558213 |
Target: 5'- aGC-UCGGCGaGCUGCGgccCGGuGAGGCCg -3' miRNA: 3'- -CGuGGCUGC-CGGUGUa--GCCcUUCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 9931 | 0.66 | 0.557156 |
Target: 5'- -aGCCccCGGCCGuccagguCAUCGGGGAGcGCa -3' miRNA: 3'- cgUGGcuGCCGGU-------GUAGCCCUUC-CGg -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 11806 | 0.66 | 0.557156 |
Target: 5'- uGUA-CGACGGCCcUGUCGGcguccacGAAGGCa -3' miRNA: 3'- -CGUgGCUGCCGGuGUAGCC-------CUUCCGg -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 3459 | 0.66 | 0.55188 |
Target: 5'- gGCACCGAcacccCGGCCAUcgacuacaacgacguGUCcgagaacccgcaGGGucuGGCCg -3' miRNA: 3'- -CGUGGCU-----GCCGGUG---------------UAG------------CCCuu-CCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 45416 | 0.66 | 0.54767 |
Target: 5'- cGUACCuGAagccuccacCGGCCAUGcCGGGGuaGGGCUc -3' miRNA: 3'- -CGUGG-CU---------GCCGGUGUaGCCCU--UCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 27187 | 0.66 | 0.54767 |
Target: 5'- cGCAuCUGACGGuCCuccuCuacCGGGA-GGCCc -3' miRNA: 3'- -CGU-GGCUGCC-GGu---Gua-GCCCUuCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 24038 | 0.66 | 0.53719 |
Target: 5'- aCGCC--UGGCCACGggcacCGGGAccaccGGCCa -3' miRNA: 3'- cGUGGcuGCCGGUGUa----GCCCUu----CCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 3068 | 0.66 | 0.53719 |
Target: 5'- cCGCCGAC--CCGaccggCGGGAAGGUCg -3' miRNA: 3'- cGUGGCUGccGGUgua--GCCCUUCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 17244 | 0.66 | 0.526781 |
Target: 5'- cGCACUGGCGaGUUcuuCAcCGGGcucGGGCCg -3' miRNA: 3'- -CGUGGCUGC-CGGu--GUaGCCCu--UCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 38894 | 0.67 | 0.516448 |
Target: 5'- gGCcCUGAUGGCCuCAgCGG--AGGCCg -3' miRNA: 3'- -CGuGGCUGCCGGuGUaGCCcuUCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 4954 | 0.67 | 0.506198 |
Target: 5'- uCACCGAgGaguuCCACAgggUCGaGGAAGGCa -3' miRNA: 3'- cGUGGCUgCc---GGUGU---AGC-CCUUCCGg -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 19582 | 0.67 | 0.506198 |
Target: 5'- cGCugCGGaucaagguCGGCCGCGagcaGGGcGGGCUc -3' miRNA: 3'- -CGugGCU--------GCCGGUGUag--CCCuUCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 33303 | 0.67 | 0.505177 |
Target: 5'- aGC-UCGACGGUCACAucuUCGGaGAucccgaucgugacGGGCUc -3' miRNA: 3'- -CGuGGCUGCCGGUGU---AGCC-CU-------------UCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 6330 | 0.67 | 0.496035 |
Target: 5'- cGCACCGgccACGGCCGCuGUC---AAGGUCa -3' miRNA: 3'- -CGUGGC---UGCCGGUG-UAGcccUUCCGG- -5' |
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7167 | 3' | -58.2 | NC_001900.1 | + | 15281 | 0.67 | 0.496035 |
Target: 5'- -gGCCG-UGGCCGCA-CGGuucGGCCg -3' miRNA: 3'- cgUGGCuGCCGGUGUaGCCcuuCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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