miRNA: 3'- aaCGACGGAGCU--ACgCCGGU-------GGC-CGg -5'
miRNA: 3'- aACGa----CGGAgCUACgCCGG-----UG-GCCGG- -5'
miRNA: 3'- aaCGaCGgAGCUA-CGC--------CGGUGGCCGG- -5'
miRNA: 3'- aaCGAC-GGag-CUACGcCGGUGGCCGG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7170 | 3' | -61.6 | NC_001900.1 | + | 42955 | 0.68 | 0.263722 |
|
Target: 5'- -gGCUGCCUCGAucUG-GGCCAgcacguuCCGaGCg -3' miRNA: 3'- aaCGACGGAGCU--ACgCCGGU-------GGC-CGg -5' |
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| 7170 | 3' | -61.6 | NC_001900.1 | + | 43751 | 0.67 | 0.291974 |
|
Target: 5'- gUGCagucgGCCUgGAUGaGGCCgucacGCaCGGCCg -3' miRNA: 3'- aACGa----CGGAgCUACgCCGG-----UG-GCCGG- -5' |
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| 7170 | 3' | -61.6 | NC_001900.1 | + | 45541 | 0.69 | 0.204658 |
|
Target: 5'- -cGCcGCgUCGAUgGCGuccuggauGUCACCGGCCa -3' miRNA: 3'- aaCGaCGgAGCUA-CGC--------CGGUGGCCGG- -5' |
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| 7170 | 3' | -61.6 | NC_001900.1 | + | 47441 | 0.75 | 0.080989 |
|
Target: 5'- -cGCUGaCCgggGGUGUcGCCACCGGCCg -3' miRNA: 3'- aaCGAC-GGag-CUACGcCGGUGGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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